黃芳

1984年和1989年在蘭州大學獲學士和碩士學位。2003年在瑞典Stockholm University獲博士學位。1992-1994年，在英國Horticultural Research International(HRI)進行訪問研究。2003-2004年在瑞典Stockholm University做Research Fellow。2005年入選中國科學院“百人計劃”。任中科院植物研究所光合作用功能蛋白質組學創新研究組組長。《植物學報》編委。近年來，在PNAS等國際學術刊物發表論文十餘篇。培養具有碩士和博士學位的研究生6名、博士後1名；正在培養的博士和碩士研究生9名。 ^[2] 

主要研究工作為藍藻光合系統的生物發生機制與調控、綠藻光合作用與光合產氫的生物發生機制與調控、光合微藻逆境適應分子機制。

1. 藍藻光合系統的生物發生機制與調控

主要以光合模式藍藻（如Synechocystis sp. PCC 6803）為材料，利用生物化學、分子遺傳學和光譜學等手段，探討光合膜系統及膜蛋白系統演化與功能調控的分子機理。

2．綠藻光合作用與光合產氫的生物發生機制與調控

主要以光合作用與光合產氫的模式綠藻（如Chlamydomonas reinhardtii）為材料，採用蛋白質組學和分子遺傳學手段，探討光合作用與光合產氫的代謝網絡調控規律並發現重要基因靶標, 培育高光效產氫微藻株系。

3．光合微藻逆境適應分子機制

主要從細胞和亞細胞水平，研究光合微藻對逆境（如高光強、高鹽鹼等）的分子適應機制，發展微藻資源高效利用的途徑和方法。

[1] “萊茵衣藻e6突變體基因及其在光能轉化中的作用”, 國家自然科學基金，2013.1-2016.12（主持）。

[2] “衣藻乙酸代謝突變體ACM1基因克隆及其在光合產氫中的作用”，國家自然科學基金，2012.1-2015.12（主持）。

[3] “高效低成本直接太陽能化學及生物轉化與利用的基礎研究”，國家重點基礎研究發展計劃(973)，2009.1-2013.12，第七課題（主持）。

[4] “提高作物養分與光合效率基因的克隆與功能驗證”, 國家轉基因重大專項項目，2009.1-2011.12（主持）。

[5] “基於光合作用原理的微藻水光解制氫研究”，中國科學院重要方向項目， 2009.1-2011.12（主持）。

[6] “藍藻重要鹽脅迫誘導表達蛋白生物學功能研究”，國家自然科學基金，2008.1-2010.12（主持）。

[7] “特殊生境植物資源的開發利用技術”, 國家高技術研究發展計劃（863）重點項目, 2007.1-2010.12（參加）。

[8] “微藻光合放氫的生物學基礎及調控途徑”, 中國科學院重要方向項目， 2009.6-2010.12（參加）。

[9]“單細胞藻類質膜應答極端pH及其它逆境的蛋白組學研究”, 國家自然科學基金，2007.1-2007.12（主持）。

[10] “涉入藻類產氫的蛋白質分子鑑定及其功能研究”，國家自然科學基金，2006.1-2008.12（主持）。

[11] “藻類產氫分子調控機理”，國家人事部留學人員擇優重點項目，2007.1-2007.12（主持）。

[1] 黃芳，楊浩萌，趙樂，薛哲勇，周荷益，漆小泉; 一種集胞藻蛋白在改良水稻性狀中的新用途; ZL201110270947.0; 2014.02.05

[2] 黃芳，楊浩萌，趙樂，薛哲勇，周荷益，漆小泉; 一種與二氧化碳濃縮機制相關的集胞藻蛋白的新用途; ZL2011102709517; 2014.05.14

[3] 黃芳，吳驪珠，王紅豔，陳梅，孫永樂，趙磊，陳彬; 一種利用光合產氫微藻高效篩選放氫催化劑的方法; ZL201110100773.3; 2014.01.15

[4] 黃芳，趙磊; 提高衣藻產氫量的培養基及其製備方法; ZL201110230908.8; 2014.05.07

[5] 黃芳，楊浩萌，趙樂，薛哲勇，周荷益，漆小泉; 一種集胞藻蛋白的新用途; ZL201110258388.1; 2014.06.18

[6] 黃芳，趙磊，楊浩萌; 抗氧化及營養脅迫相關蛋白及其編碼基因與應用; ZL201210479314.5; 2016.01.13

[7] 黃芳，趙磊，程冬梅; 具有光保護功能的蛋白及其編碼基因與應用; ZL201210479164.8; 2016.01.13

[8] 黃芳，孫永樂，趙樂，楊浩萌，陳梅; 一種生物鎘抗性相關蛋白及其編碼基因; ZL20090923926.9; 2012.08.29

[9] 黃芳，陳梅，孫永樂; 產氫量提高的基因工程衣藻及其應用; ZL201110045287.6; 2012.10.10

[10] 黃芳，趙磊，陳梅，孫永樂; 一種高效篩選衣藻放氫突變體的方法; ZL201010233108.7; 2013.05.29

[11] 黃芳，趙磊，楊浩萌，陳梅; 用於擴增產氫微藻萊茵衣藻插入突變基因側翼序列的引物及方法; ZL201110026604.X; 2013.09.04

[12] 黃芳，趙磊，陳梅，張錦; 一株超高放氫藻株及其應用; ZL201410014492.X; 2016.03.02 ^[1-2] 

Chen M, Liu P, Zhang J, Peng LW, Huang F*. 2019. Photochemical characteristics of Chlamydomonas mutant hpm91 lacking Proton Gradient Regulation 5 (PGR5) during sustained H₂ photoproduction under sulfur deprivation. J. International Journal of Hydrogen Energy, 44: 31790-31799.

Li Lingyu, Yang Haomeng, Peng Lianwei, Ren Weibo, Gong Jirui, Liu Peng, Wu Xinhong, Huang Fang*. 2019. Comparative study reveals insights of sheepgrass (Leymus chinensis) coping with phosphate-deprived stress condition. Front Plant Sci., 10:170.

Li LY, Yang HM, Liu P, Ren WB, Wu XH, Huang F*. 2018. Combined impact of heat stress and phosphate deficiency on growth and photochemical activity of sheepgrass (Leymus chinensis). J. Plant Physiol., 231: 271-276.

Zhao L, Cheng DM, Huang XH, Chen M, Dall’Osto L, Xing JL, Gao LY, Li LY, Wang YL, Bassi R, Peng LW, Wang YC, Rochaix J-D, Huang F*. 2017. A light harvesting complex-like protein in maintenance of photosynthetic components in Chlamydomonas. Plant Physiol., 174: 2419-2433.

Xing J, Liu P, Zhao L, Huang F*. 2017. Deletion of CGLD1 impairs PSII and increases singlet oxygen tolerance of green alga Chlamydomonas reinhardtii. Front. Plant Sci., 8:2154

Chen Mei, Zhang Jin, Zhao Lei, Xing Jiale, Peng Lianwei, Kuang Tingyun, Rochaix Jean-David, Huang Fang*. 2016. Loss of algal Proton Gradient Regulation 5 increases reactive oxygen species scavenging and H2 evolution. Journal of Integrative Plant Biology, 58(12): 943-946.

Li Lingyu, Yang Haomeng, Ren Weibo, Liu Bei, Cheng Dongmei, Wu Xinghong, Gong Jirui, Peng Lianwei, Huang Fang*. 2016. Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions. Journal of Plant Biology, 59: 336-346.

Chen Mei, Zhang Jin, Zhao Lei, Xing Jiale, Peng Lianwei, Kuang Tingyun, Rochaix Jean-David, Huang Fang*. 2016. Loss of algal proton gradient regulation 5 increases ros scavenging and h 2 evolution: h 2 production of chlamydomonas mutant lacking pgr5. Plant Physiology, 58(12), 943-946.

Li Li, Deng Dandan, Chen Xi, Wu Baomei, Hu Ke, Qiu Tianhang, Cui Suxia*, Huang Fang*. 2015. Expression of the moss PpLEA4-20 gene in rice enhances membrane protection and client proteins stability. Biochemical and Biophysical Research Communications, 460(2): 386-391.

Gao LY, Shen CT, Liao LB, Huang XH, Liu KH, Wang W, Guo LH, Jin WH, Huang F, Xu W, Wang YC. 2014. Functional proteomic discovery of Slr0110 as a central regulator of carbohydrate metabolism in Synechocystis sp. PCC 6803. Molecular & Cellular Proteomics, 13:204-219.

Yang HM, Liao LB, Bo TT, Zhao L, Sun XW, Lu XF, Norling B, Huang F.* 2014. Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition. Journal of Integrative Plant Biology, 56:1136-1150.

Zhao L, Chen M, Cheng DM, Yang HM, Sun YL, Zhou HY, Huang F.* 2013. Different B-type Methionine Sulfoxide Reductases in Chlamydomonas may Protect the Alga against High-light, Sulfur-depletion or Oxidative Stress. Journal of Integrative Plant Biology, 55: 1053-1067.

楊浩萌, 薛哲勇, 趙樂, 漆小泉, 黃芳*. 2013. 集胞藻slr1515基因提高轉基因水稻植株劍葉長度及葉綠素含量. 植物學報, 48卷 5期 489-497頁.

楊浩萌, 薛哲勇, 趙樂, 漆小泉, 黃芳*. 2013. 集胞藻sbtA基因具有提高水稻旗葉葉綠素含量及株高的功能. 植物生理學報 , 9卷 4期 351-356頁.

Sun YL, Chen M, Yang HM, Zhang J, Kuang TY, Huang F.* 2013. Enhanced H₂ photoproduction by down-regulation of ferredoxin-NADP+ reductase (FNR) in the green alga Chlamydomonas reinhardtii. International Journal of Hydrogen Energy. 38: 16029-16037.

Li T, Yang HM, Cui SX, Suzuki I, Zhang LF, Li L, Bo TT, Wang J, Murata N, Huang F.* 2012. Proteomic study of the impact of Hik33 mutation in Synechocystissp. PCC 6803 under normal and salt stress conditions. Journal of Proteome Research, 1: 502-514

Zhao L, Sun YL, Cui SX, Chen M, Yang HM, Liu HM, Chai TY, Huang F.* 2011. Cd-induced proteomic changes in hyperaccumulator Phytolacca americana. Chemosphere, 85: 56-66

Chen M, Zhao L, Sun YL, Cui SX, Zhang LF, Yang B, Wang J, Kuang TY, Huang F.* 2010. Proteomic analysis of hydrogen photoproduction in sulfur-deprived Chlamydomonas cells. Journal of Proteome Research, 9: 3854-3866.

Zhang LF, Yang HM, Cui SX, Hu J, Wang J, Kuang TY, Norling B. Huang F.* 2009. Proteomic analysis of plasma membranes of cyanobacterium Synechocystis sp. strain PCC 6803 in response to high pH stress. Journal of Proteome Research, 8: 2892-2902.

Cui SX, Hu J, Yang B, Shi L, Huang F, Tsai S, He YK. Zhang J. 2009. Proteomic characterization of Phragmites communis in ecotypes of swamp and desert dune. Proteomics, 9: 3950–3967.

Zhou JX, Zhou J, Yang HM, Chen M. Huang F.* 2008. Characterization of two glutaminases from the filamentous cyanobacterium Anabaena sp. PCC 7120. FEMS Microbiology Letters, 289: 241-249.

Zhou J, Zhou JX, Yang HM, Yan CS, Huang F.* 2008. Characterization of a sodium-regulated glutaminase from cyanobacterium Synechocystis sp. PCC 6803. Science in China Series C: Life Sciences, 51: 1066-1075.

Rajalahti T, Huang F, Klement M, Pisareva T, Edman M, Sjostrom M, Wieslander A. Norling, B. 2007. Proteins in different Synechocystis compartments have distinguishing N-terminal features: a combined proteomics and multivariate sequence analysis. Journal of Proteome Research, 7: 2420-2434.

Ran L, Huang F, Ekman M, Klint J, Bergman B. 2007. Proteomic analyses of the photoauto- and diazotrophically grown cyanobacterium Nostoc sp. PCC 73102. Microbiology-SGM, 153: 608–618.

Huang F, Fulda S, Hagemann M, Norling B. 2006. Proteomic screening of salt-stress-induced changes in plasma membranes of Synechocystis sp. strain PCC 6803. Proteomics, 6: 910-920.

Fulda S, Mikkat S, Huang F, Huckauf J, Marin K, Norling B, Hagemann M. 2006. Proteome analysis of salt stress response in the cyanobacterium Synechocystis sp. strains PCC 6803. Proteomics, 6: 2733-2745.

Huang F, Hedman E, Funk C, Kieselbach T, Schroder PW, Norling B. 2004. Isolation of outer membrane of Synechocystis sp. PCC 6803 and its proteomic characterization. Molecular & Cellular Proteomics, 3: 586-595

Huang F, Parmryd I, Nilsson, F, Persson AL, Pakrasi HB, Andersson B, Norling B. 2004. Proteomics of Synechocystis sp strain PCC 6803 - Identification of plasma membrane proteins. Molecular & Cellular Proteomics, 1: 956-966

Zak E, Norling B, Maitra R, Huang F, Andersson B, Pakrasi HB. 2001. The initial steps of biogenesis of cyanobacterial photosystems occur in plasma membranes. Proceedings of the National Academy of Sciences of the United States of America, 98: 13443-13448.

Fulda S, Huang F, Nilsson F, Hagemann M, Norling B. 2000. Proteomics of Synechocystis sp strain PCC 6803-Identification of periplasmic proteins in cells grown at low and high salt concentrations. European Journal of Biochemistry, 267: 5900-5907

Huang F, Zhang XP, Szigyarto C, Glaser E. 1998. Import and processing of nuclear-encoded mitochondrial proteins during development of pea plants. In Plant Mitochondria: From Gene to Function. (I.M. Moller, P. Gardestrom, K. Glimelius and E. Glaser, eds.). Backhuys Publishers, Leiden, The Netherlands, pp 267-271.

Wen JQ, Huang F, Liang WS, Liang HG. 1997. Increase of HCN and beta-cyanoalanine synthase activity during ageing of potato tuber slices. Plant Science, 125: 147-151

文江祁, 黃芳, 梁厚果. 1997. 馬鈴薯塊莖HCN的釋放與CAS活性變化. 科學通報, 24: 8-20. ^[2]