關怡新

1989年7月本科畢業於清華大學化工系，

1995年1月研究生畢業於浙江大學化工系，獲工學博士學位。

1995年3月留校在浙江大學化工系任教至今，

1999年8月至2000年7月受包兆龍包玉剛中國留學生獎學金資助英國劍橋大學蛋白質工程中心訪問學者， 2005年12月晉升為教授，

2006年9月批准為博士生導師 ^[1] 

· 生物製藥技術

· 生物催化和轉化

· 蛋白質工程

主要從事生物化工領域的教學和科研工作。

研究興趣

● 超臨界流體技術在生物醫藥領域的應用

超臨界微粒化過程用於藥物劑型，超臨界浸漬過程負載藥物實現緩控釋給藥，超臨界發泡法制備多孔組織工程支架，天然產物的超臨界提取技術。

● 蛋白質體內外摺疊技術及機理

分子伴侶共表達促進重組蛋白質可溶表達，摺疊酶介導的蛋白質體內外摺疊路徑及其機理，智能型聚合物介導的基因工程蛋白質體外復性過程和新型集成復性技術。

● 甾體藥物的微生物轉化

開發高附加值甾體藥物的生物和化學協同催化合成新工藝，包括微生物羥化、脱氫和甾體側鏈降解等生物催化和轉化過程。 ^[1] 

1、生化反應工程（雙語），浙江省精品課程，本科生，1996-現在

2、生物化工專題討論（專業外語），博士生，2005-現在 ^[1] 

作為項目負責人承擔國家自然科學基金7項，教育部博士點基金1項，浙江省自然科學基金1項，浙江省科技計劃項目1項，並參與了多項國家自然科學基金重點項目和國際合作項目。研究成果獲教育部科技進步一等獎和浙江省科學技術二等獎。主要成果發表在 BIOTECHNOL BIOENG, BIORESOURCE TECHNOL, APPL MICROBIOL BIOT, BIOCHEM ENG J, CHEM ENG SCI, IND ENG CHEM RES, J SUPERCRIT FLUID, J MEMBRANE SCI, J CHROMATOGR A, INT J PHARMACEUT, ACS SUSTAIN CHEM ENG 等top期刊，共發表SCI收錄論文87篇，出版學術著作1部，授權國家發明專利24項。 ^[1] 

1、 強化兩相混合的超臨界輔助霧化法制備蛋白類藥物微粒應用基礎研究，高等學校博士學科點專項科研基金

2、 含離子液體體系中生物轉化法制備藥物中間體雄烯二酮應用基礎研究，國家自然科學基金

3、 超臨界流體技術在水體系中製備藥用蛋白質及複合微粒的應用基礎研究，國家自然科學基金

4、 超臨界流體技術製備多孔組織工程支架的應用基礎研究，國家自然科學基金

5、 超臨界溶液浸漬法制備緩控釋藥物微粒的應用基礎研究，國家自然科學基金

6、 接枝型智能聚合物介導的蛋白質體外摺疊過程基礎研究，國家自然科學基金

7、 水力空化增強超臨界輔助霧化製備氣溶膠藥物微粒，國家自然科學基金

8、 摺疊酶介導的蛋白質體外摺疊過程基礎研究，國家自然科學基金

9、 雙丙酸倍氯米松生產工藝開發，浙江省科技計劃項目

10、 6α-甲基強的松龍生產工藝開發，寧波市科技計劃項目

11、 分子伴侶純化及對粒細胞集落刺激因子復性的作用，浙江省自然科學基金

12、 重組蛋白的新型復性及純化技術，教育部留學回國人員科研啓動基金 ^[1] 

(1) Peng Huhong, Hong Dongxiao, Guan Yixin*, Yao Shanjing, Preparation of pH-responsive DOX-loaded chitosan nanoparticles using supercritical assisted atomization with an enhanced mixer,,, 558, 82-90 International Journal of Pharmaceutics 2019

(2) Wu Qingxi, Guan Yixin, Yao Shanjing, Sodium cellulose sulfate: A promising biomaterial used for microcarriers' designing, ,, 13, 46-58Frontiers of Chemical Science and Engineering 2019

(3) Guan Jintao, Han Chaofei, Guan Yixin*, Zhang Songhong, Yun Junxian*, Yao Shanjing, Optimizational production of phenyllactic acid by a Lactobacillus buchneri strain via uniform design with overlay sampling methodology, , , 27, 418-425ChineseJournal of Chemical Engineering2019

(4) Chen Chuanxin, Peng Huhong, Guan Yixin*, Yao Shanjing, Morphological study on the pore growth profile of poly(ε-caprolactone) bi-modal porous foams using a modified supercritical CO2 foaming process, The Journal of Supercritical Fluids, 2019, 143, 72-81

(5) Hong Dongxiao, Yun Yulong, Guan Yixin*, Yao Shanjing, Preparation of micrometric powders of parathyroid hormone (PTH1-34)-loaded chitosan oligosaccharide by supercritical fluid assisted atomization, International Journal of Pharmaceutics, 2018, 545, 389-394

(6) Guan Jintao, Guan Yixin*, Yun Junxian*, Yao Shanjing, Chromatographic separation of phenyllactic acid from crude broth using cryogels with dual functional groups, Journal of Chromatography A, 2018, 1554, 92-100

(7) Xin Xin, Guan Yixin*, Yao Shanjing, Sustained release of dexamethasone from drug-loading PLGA scaffolds with specific pore structure fabricated by supercritical CO2 foaming, Journal of Applied Polymer Science, 2018, 135, 46207

(8) Xin Xin, Guan Yixin*, Yao Shanjing, Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO2 foaming, Chinese Journal of Chemical Engineering, 2018, 26, 207-212

(9) Yuan Junjie, Guan Yixin*, Yao Shanjing, Evaluation of biocompatible ionic liquids for their application in phytosterols bioconversion by Mycobacterium sp. resting cells, ACS Sustainable Chemistry & Engineering, 2017, 5, 10702-10709

(10) Chen Chuanxin, Liu Qianqian, Xin Xin, Guan Yixin*, Yao Shanjing, Pore formation of poly(ε-caprolactone) scaffolds with melting point reduction in supercritical CO2 foaming, The Journal of Supercritical Fluids, 2016, 117, 279-288

(11) Yuan Junjie, Guan Yixin*, Wang Yanting, Wang Haiqing, Yao Shanjing, Side-chain cleavage of phytosterols by Mycobacterium sp. MB 3683 in a biphasic ionic liquid/aqueous system, Journal of Chemical Technology and Biotechnology, 2016, 91, 2631-2637

(12) Xin Xin, Liu Qianqian, Chen Chuanxin, Guan Yixin*, Yao Shanjing, Fabrication of bimodal porous PLGA scaffolds by supercritical CO2 foaming/particle leaching technique, Journal of Applied Polymer Science, 2016, 33, 43644

(13) Shen Yubin, Du Zhe, Tang Chuan, Guan Yixin, Yao Shanjing, Formulation of insulin-loaded N-trimethyl chitosan microparticles with improved efficacy for inhalation by supercritical fluid assisted production, International Journal of Pharmaceutics, 2016, 505, 223-233

(14) Shen Yubin, Guan Yixin, Yao Shanjing, Supercritical fluid assisted production of micrometric powders of the labile trypsin and chitosan/trypsin composite microparticles, International Journal of Pharmaceutics, 2015, 489, 226-236

(15) Yun Junxian, Cheng Xiuhong, Ye Jialei, Shen Shaochuan, Yang Gensheng, Yao Kejian, Harald Kirsebom, Lin Dongqiang, Guan Yixin, Yao Shanjing, Chromatographic adsorption of serum albumin and antibody proteins in cryogels with benzyl-quaternary amine ligands, Journal of Chromatography A, 2015, 1381, 173-183

(16) Zhuo Xiaofa, Zhang Yiying, Guan Yixin*, Yao Shanjing, Co-expression of disulfide oxidoreductases DsbA/DsbC markedly enhanced soluble and functional expression of reteplase in Escherichia coli, Journal of Biotechnology, 2014, 192, 197-203

(17) Zhao Wei, Zhang Songhong, Lu Meizhen, Shen Shaochuan, Yun Junxian, Yao Kejian, Xu Linhong, Lin Dongqiang, Guan Yixin, Yao Shanjing, Immiscible liquid-liquid slug flow characteristics in the generation of aqueous drops with a rectangular microchannel for preparation of poly(2-hydroxyethylmethacrylate) cryogel beads, Chemical Engineering Research and Design, 2014, 92, 2182-2190

(18) Tang Chuan, Guan Yixin*, Yao Shanjing, Zhu Ziqiang, Solubility of dexamethasone in supercritical carbon dioxide with and without a cosolvent, Journal of Chemical and Engineering Data, 2014, 59, 3359-3364

(19) Chen Huiying, Guan Yixin, Yao Shanjing, A novel two-species whole-cell immobilization system composed of marine-derived fungi and its application in wastewater treatment, Journal of Chemical Technology and Biotechnology, 2014, 89, 1733-1740

(20) Tang Chuan, Guan Yixin*, Yao Shanjing, Zhu Ziqiang, Preparation of ibuprofen-loaded chitosan films for oral mucosal drug delivery using supercritical solution impregnation, International Journal of Pharmaceutics, 2014, 473, 434-441

(21) Xu Yangguang, Guan Yixin*, Wang Haiqing, Yao Shanjing, Microbial side-chain cleavage of phytosterols by Mycobacteria in vegetable oil/aqueous two-phase system, Applied Biochemistry and Biotechnology, 2014, 174, 522-533

(22) Shen Yubin, Du Zhe, Wang Qi, Guan Yixin, Yao Shanjing, Preparation of chitosan microparticles with diverse molecular weights using supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer, Powder Technology, 2014, 254, 416-424

(23) Du Zhe, Shen Yubin, Tang Chuan, Guan Yixin*, Yao Shanjing, Zhu Ziqiang, Supercritical fluid assisted production of chitosan oligomers micrometric powders, Carbohydrate Polymers, 2014, 102, 400-408

(24) Ye Jialei, Yun Junxian, Lin Dongqiang, Xu Linhong, Harald Kirsebom, Shen Shaochuan, Yang Gensheng, Yao Kejian, Guan Yixin, Yao Shanjing, Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads for the separation of human serum immunoglobulin and albumin, Journal of Separation Science, 2013, 36, 3813-3820

(25) Yu Jinpeng, Tang Chuan, Guan Yixin*, Yao Shanjing, Zhu Ziqiang, Sorption and diffusion behavior of carbon dioxide into poly(l-lactic acid) films at elevated pressures, Chinese Journal of Chemical Engineering, 2013, 21, 1296-1302

(26) Du Zhe, Tang Chuan, Guan Yixin*, Yao Shanjing, Zhu Ziqiang, Bioactive insulin microparticles produced by supercritical fluid assisted atomization with an enhanced mixer, International Journal of Pharmaceutics, 2013, 454, 174-182

(27) Huang Shoukun, Jin Jiayu, Guan Yixin*, Yao Zhen, Cao Kun, Yao Shanjing, Refolding of recombinant human interferon gamma inclusion bodies in vitro assisted by colloidal thermo-sensitive poly(N-isopropylacrylamide) brushes grafted onto the surface of uniform polystyrene cores, Biochemical Engineering Journal, 2013, 74, 20-26

(28) Luo Man, Guan Yixin*, Yao Shanjing, Optimization of DsbA purification from a recombinant Escherichia coli broth using Box-Behnken design methodology, Chinese Journal of Chemical Engineering, 2013, 21, 185-191

(29) Tong Shengqiang, Zheng Ye, Yan Jizhong, Guan Yixin, Wu Chunyan, Lei Wenyu, Preparative enantioseparation of β-blocker drugs by counter-current chromatography using dialkyl l-tartrate as chiral selector based on borate coordination complex, Journal of Chromatography A, 2012, 1263, 74-83

(30) Xue Dongsheng, Chen Huiying, Lin Dongqiang, Guan Yixin, Yao Shanjing, Optimization of a natural medium for cellulase by a marine Aspergillus niger using response surface methodology, Applied Biochemistry and Biotechnology, 2012, 167, 1963-1972

(31) Luo Man, Guan Yixin*, Yao Shanjing, Statistical optimization and multiple objective programming of lysozyme refolding catalyzed by recombinant DsbA in vitro, Process Biochemistry, 2012, 47, 1268-1276

(32) Yun Junxian, Tu Changming, Lin Dongqiang, Xu Linhong, Guo Yantao, Shen Shaochuan, Zhang Songhong, Yao Kejian, Guan Yixin, Yao Shanjing, Microchannel liquid-flow focusing and cryo-polymerization preparation of supermacroporous cryogel beads for bioseparation, Journal of Chromatography A, 2012, 1247, 81-88

(33) Wang Qi, Guan Yixin, Yao Shanjing, Zhu Ziqiang, The liquid volume expansion effect as a simple thermodynamic criterion in cholesterol micronization by supercritical assisted atomization, Chemical Engineering Science, 2012, 75, 38-48

(34) Yan Xiao, Hu Sheng, Guan Yixin*, Yao Shanjing, Coexpression of chaperonin GroEL/GroES markedly enhanced soluble and functional expression of recombinant human interferon-gamma in Escherichia coli, Applied Microbiology and Biotechnology, 2012, 93, 1065-1074

(35) Yu Jinpeng, Guan Yixin*, Yao Shanjing, Zhu Ziqiang, Preparation of roxithomycin-loaded poly (l-lactic acid) films with supercritical solution impregnation, Industrial & Engineering Chemistry Research, 2011, 50, 13813-13818

(36) Du Zhe, Guan Yixin*, Yao Shanjing, Zhu Ziqiang, Supercritical assisted atomization introduced by an enhanced mixer for micronization of lysozyme: particle morphology, size and protein stability, International Journal of Pharmaceutics, 2011, 421, 258-268

(37) Luo Man, Guan Yixin*, Yao Shanjing, On-column refolding of denatured lysozyme by the combined chromatography composed of SEC and immobilized recombinant DsbA, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2011, 879, 2971-2977

(38) Wu Dongxiang, Guan Yixin*, Wang Haiqing, Yao Shanjing, 11α-Hydroxylation of 16α,17-epoxyprogesterone by Rhizopus nigricans in a biphasic ionic liquid aqueous system, Bioresource Technology, 2011, 102, 9368-9373

(39) Jin Jiayu, Guan Yixin*, Yao Shanjing, Effect of the SA content of a novel thermo-sensitive P(NIPAM-co-SA) copolymer on denatured lysozyme refolding in vitro, Journal of Applied Polymer Science, 2011, 121, 2597-2605

(40) Tong Shengqiang, Yan Jizhong*, Guan Yixin*, Lu Youming, Enantioseparation of phenylsuccinic acid by high speed counter-current chromatography using hydroxypropyl-β-cyclodextrin as chiral selector, Journal of Chromatography A, 2011, 1218, 5602-5608

(41) Tong Shengqiang, Guan Yixin*, Yan Jizhong*, Zheng Bei, Zhao Liying, Enantiomeric separation of (R,S)-naproxen by recycling high speed counter-current chromatography with hydroxypropyl-β-cyclodextrin as chiral selector, Journal of Chromatography A, 2011, 1218, 5434-5440

(42) Wang Qi, Guan Yixin, Yao Shanjing, Zhu Ziqiang, Controllable preparation and formation mechanism of BSA microparticles using supercritical assisted atomization with an enhanced mixer, The Journal of Supercritical Fluids, 2011, 56, 97-104

(43) Miao Shifeng, Yu Jinpeng, Du Zhe, Guan Yixin*, Yao Shanjing, Zhu Ziqiang, Supercritical fluid extraction and micronization of ginkgo flavonoids from ginkgo biloba leaves, Industrial & Engineering Chemistry Research, 2010, 49, 5461-5466

(44) Wang Qi, Guan Yixin, Yao Shanjing, Zhu Ziqiang, Microparticle formation of sodium cellulose sulfate using supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer, Chemical Engineering Journal, 2010, 159, 220-229

(45) Tong Shengqiang, Yan Jizhong*, Guan Yixin*, Fu Yaner, Yoichiro Ito, Separation of α- cyclohexylmandelic acid enantiomers using biphasic chiral recognition high-speed counter-current chromatography, Journal of Chromatography A, 2010, 1217, 3044-3052

(46) Ge Xiang, Guan Yixin, Chen Jie, Yao Zhen, Cao Kun, Yao Shanjing, Refolding of lysozyme in vitro assisted by colloidal thermo-sensitive poly(N-isopropylacrylamide) brushes grafted onto the surface of uniform polystyrene cores, Journal of Applied Polymer Science, 2009, 114, 1270-1277

(47) Li Jiuhong, Guan Yixin*, Wang Haiqing, Yao Shanjing, Dehydrogenation of 11α-hydroxy-16α,17-epoxyprogesterone by encapsulated Arthrobacter simplex cells in an organic-aqueous two-liquid-phase system, Journal of Chemical Technology and Biotechnology, 2009, 84, 208-214

(48) Ni Hongliang, Guan Yixin, Yao Shanjing, A combined process of biocatalysis and cell activity regeneration for the asymmetric reduction of 3-oxo ester with immobilized baker's yeast, Journal of Chemical Technology and Biotechnology, 2009, 84, 186-191

(49) Tong Shengqiang, Yan Jizhong*, Guan Yixin*, Preparative separation of isomeric caffeoylquinic acids from Flos Lonicerae by pH-zone-refining counter-current chromatography, Journal of Chromatography A, 2008, 1212, 48-53

(50) Xu Liren, Yan Xiao, Luo Man, Guan Yixin*, Yao Shanjing, Preparation, characterization and refolding in vitro of a recombinant human cyclophilin A mutant: effect of a single pro/ser substitution on cyclophilin A structure and properties, Biotechnology Progress, 2008, 24, 302-310

(51) Cai Meiqiang, Guan Yixin, Yao Shanjing, Zhu Ziqiang, Supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer (SAA-HCM) for micronization of levofloxacin hydrochloride, The Journal of Supercritical Fluids, 2008, 43, 524-534

(52) Wu Yin, Yao Shanjing, Guan Yixin, Inactivation of microorganisms in carbon dioxide at elevated pressure and ambient temperature, Industrial & Engineering Chemistry Research, 2007, 46, 6345-6352

(53) Chen Guo, Yao Shanjing, Guan Yixin, Influence of osmoregulators on osmotolerant yeast Candida krusei for the production of glycerol, Chinese Journal of Chemical Engineering, 2006, 14, 371-376

(54) Zhang Jun, Guan Yixin, Ji Zheng, Yao Shanjing, Effects on membrane properties of NaCS-PDMDAAC capsules by adding inorganic salts, Journal of Membrane Science, 2006, 277, 270-276

(55) Jin Ting, Guan Yixin, Yao Shanjing, Lin Dongqiang, Cho Mangi, On-column refolding of recombinant human interferon-γ inclusion bodies by expanded bed adsorption chromatography, Biotechnology and Bioengineering, 2006, 93, 755-760

(56) Yao Shanjing, Guan Yixin, Lin Dongqiang, Mass transfer behavior of solutes in NaCS-PDMDAAC capsules, Industrial & Engineering Chemistry Research, 2006, 45,1811-1816

(57) Guan Yixin*, Fei Zhengzheng, Luo Man, Jin Ting, Yao Shanjing, Chromatographic refolding of recombinant human interferon gamma by an immobilized sht GroEL191-345 column, Journal of Chromatography A, 2006, 1107, 192-197

(58) Chen Guo, Yao Shanjing, Guan Yixin, Lin Dongqiang, Preparation and characterization of NaCS-CMC/PDMDAAC capsules, Colloids and Surfaces B: Biointerfaces, 2005,45,136-143

(59) Jin Ting, Guan Yixin, Fei Zhengzheng, Yao Shanjing, Cho Mangi, A combined refolding technique for recombinant interferon-γ inclusion bodies by ion-exchange chromatography with a urea gradient, World Journal of Microbiology and Biotechnology, 2005, 21, 797-802

(60) Yang Zhonghua, Yao Shanjing, Guan Yixin, A complex process of asymmetric synthesis of β-hydroxy ester by baker's yeast accompanied by resin adsorption, Industrial & Engineering Chemistry Research, 2005, 44, 5411-5416

(61) Zhang Jun, Yao Shanjing, Guan Yixin, Preparation of macroporous sodium cellulose sulphate/ poly(dimethyldiallyl ammonium chloride) capsules and their characteristics, Journal of Membrane Science, 2005, 255, 89-98

(62) Cui Zhifang, Guan Yixin, Chen Jianliang, Yao Shanjing, Thermosensitive poly(N-isopropyl acrylamide) hydrogel for refolding of recombinant bovine prethrombin-2 from E. coli inclusion bodies, Journal of Applied Polymer Science, 2005, 96, 1734-1740

(63) Guan Yixin, Pan Haixue, Gao Yonggui, Yao Shanjing Cho Mangi, Refolding and purification of recombinant human interferon-γ expressed as inclusion bodies in Escherichia coli using size exclusion chromatography, Biotechnology and Bioprocess Engineering, 2005, 10, 122-127

(64) Wang Yajun, Yao Shanjng, Guan Yixin, Wu Tianxing, J. F. Kennedy, A novel process for preparation of (1→3)-ß-D-glucan sulfate by a heterogeneous reaction and its structural elucidation, Carbohydrate Polymers, 2005, 59, 93-99

(65) Zhang Liyang, Yao Shanjing, Guan Yixin, Effects of poly(methylene-co-guanidine) on microbial growth in an alginate/cellulose sulfate-CaCl2/poly(methylene-co-guanidine) capsule system, Process Biochemistry, 2005, 40, 189-193

(66) Guan Yixin, Fei Zhengzheng, Luo Man, Yao Shanjing, Cho Mangi, Production of minichaperone (sht GroEL191-345) and its function in the refolding of recombinant human interferon gamma, Protein and Peptide Letters, 2005, 12, 85-88

(67) Guan Yixin, Yao Shanjing, Yu lihua, Mei Lehe, Purification and stability of an antithrombus enzyme from Bacillus subtilis, Chinese Journal of Chemical Engineering, 2004, 12, 707-710

(68) Yao Shanjing, Guan Yixin, He Weizhong, Qi Xiangming, Zhu Ziqiang, Activities of several enzymes in ethanol + water at elevated pressure of carbon dioxide, Journal of Chemical and Engineering Data, 2004, 49, 1333-1339

(69) Cui Zhifang, Guan Yixin, Yao Shanjing, A temperature-sensitive hydrogel refolding system: preparation of poly(N-isopropyl acrylamide) and its application in lysozyme refolding, Chinese Journal of Chemical Engineering, 2004, 12, 556-560

(70) Qi Xiangming, Yao Shanjing, Guan Yixin, Novel isoelectric precipitation of proteins in a pressurized carbon dioxide-water-ethanol system, Biotechnology Progress, 2004, 20,1176-1182

(71) Zhang Liyang, Yao Shanjing, Guan Yixin, Diffusion characteristics of solutes with low molecular weight in sodium alginate/cellulose sulfate—CaCl2/poly(methyleneco-guanidine) capsules, Journal of Chemical Engineering Data, 2003, 48, 864-868

(72) Gao Yonggui, Guan Yixin, Yao Shanjing Cho Mangi, On-column refolding of recombinant human interferon-γ with an immobilized chaperone fragment, Biotechnology Progress, 2003, 19, 915-920

(73) Gao Yonggui, Guan Yixin, Yao Shanjing Cho Mangi, Lysozyme at high concentration by dilution and size-exclusion chromatography, Journal of Zhejiang University SCIENCE, 2003, 4, 136-141

(74) Yao Shanjing, Guan Yixin, Yu Lihua, Adsorption performance of proteins to CM sepharose FF and DEAE sepharose adsorbents, Korean Journal of Chemical Engineering, 2003, 20, 93-98

(75) Yao Shanjing, Guan Yixin, Yu Lihua, Characterization of crude cellulase from Trichoderma reesei and purification of cellulase, Chinese Journal of Chemical Engineering, 2002, 10, 723-725

(76) Gao Yonggui, Guan Yixin, Yao Shanjing Cho Mangi, Refolding of lysozyme at high concentration in batch and fed-batch operation, Korean Journal of Chemical Engineering, 2002, 19, 871-875

(77) Yan Gongxi, Zhu Ziqiang, Guan Yixin, Han Zhaoxiong, Measurement of mass transfer coefficients and their modeling in continuous counter-current aqueous two phase systems using a packed extraction column, Chinese Journal of Chemical Engineering, 1999, 7, 196-204

(78) Guan Yixin, Zhu Ziqiang, Mei Lehe, Technical aspects of extractive purification of penicillin fermentation broth by aqueous two-phase partitioning, Separation Science & Technology, 1996,31, 2589-2597

(79) Yao Shanjing, Guan Yixin, Zhu Ziqiang, Investigation of phase equilibrium for ternary systems containing ethanol, water and carbon dioxide at elevated pressures, Fluid Phase Equilibria, 1994,99, 249-259

(80) Guan Yixin, Mei Lehe, Zhu Ziqiang, Recovery of antibiotics by aqueous two-phase partition一partitioning behavior of pure acetylspiramycin solution in polyethylene glycol / potassium phosphate aqueous two-phase systems, Biotechnology Techniques, 1994,8, 491-496

(81) 王豔婷，袁俊傑，關怡新*，姚善涇，雙水相體系中分枝桿菌降解植物甾醇製備雄甾-4-烯-3,17-二酮，高校化學工程學報，2017, 31(1), 104-110

(82) 禚孝發，關怡新*，姚善涇，二硫鍵異構酶DsbC介導重組瑞替普酶在E. coli中可溶表達，高校化學工程學報，2015, 29(3), 600-606

(83) 王俊雄，關怡新*，姚善涇，重組瑞替普酶包涵體制備及其體外復性，化工學報，2015, 66(2), 709-716

(84) 唐川，關怡新*，姚善涇，朱自強，超臨界溶液浸漬法制備負載磺胺嘧啶銀不對稱殼聚糖膜傷口敷料研究，高分子學報，2014, (6), 774-781

(85) 黃壽錕，關怡新*，於洪巍，姚善涇，巰基微球協助重組γ-豬肝酯酶包涵體體外復性，化工學報，2014, 65(1), 305-312

(86) 劉倩倩，唐川，杜哲，關怡新*，姚善涇，朱自強，超臨界CO2發泡法制備PLGA多孔組織工程支架，高分子學報，2013, (2), 174-182

(87) 繆世鋒，餘金鵬，關怡新*，姚善涇，T型微通道裝置製備單分散PLGA微球，高分子學報，2012, (2), 154-159

(88) 吳冬香，李久紅，王海清，關怡新*，姚善涇，短刺小克銀漢黴菌11β羥化環氧黃體酮研究，高校化學工程學報，2012, 26(1), 77-83

(89) 餘金鵬，關怡新*，姚善涇，朱自強，改進的鋭孔法制備粒徑均一的聚乳酸微球，高分子材料科學與工程，2011, 27(9), 115-118

(90) 葛翔，金佳鈺，關怡新，姚善涇，温敏型聚合物PNIPA和P(NIPA-co-SA)的製備及其協助溶菌酶復性研究，高校化學工程學報，2010, 24(1), 99-105

(91) 關怡新*，餘金鵬，姚善涇，朱自強，超臨界溶液浸漬法制備緩釋藥物，化工學報，2010, 61(2), 269-274

(92) 蔡美強，關怡新，姚善涇，朱自強，水力空化混合強化超臨界流體輔助霧化製備羅紅黴素超細微粒，化工學報，2008, 59(2), 293-300

(93) 龔萬本，關怡新*，王海清，姚善涇，簡單節桿菌催化6α-甲基醋酸可的松C1, 2脱氫動力學，高校化學工程學報，2007, 21(6), 983-988

(94) 羅曼，關怡新*，姚善涇，大腸桿菌二硫鍵形成蛋白A（DsbA）研究進展，生物工程學報，2007, 23(1), 7-15

(95) 應小姣，姚善涇，關怡新，高壓CO2條件下粗狀假絲酵母催化苯乙酮不對稱還原合成α-苯乙醇，催化學報，2006, 27(7), 631-635

(96) 王嘉，關怡新*，王海清，姚善涇，擬結晶投料環氧黃體酮犁頭黴羥化過程研究，生物工程學報，2006, 22(4), 662-666

(97) 關怡新，潘海學，姚善涇，疏水層析法復性重組人γ-干擾素，化工學報，2005, 56(6), 1076-1080

(98) 羅曼，關怡新*，姚善涇，重組二硫鍵異構酶在大腸桿菌中表達條件的統計優化，中國生物化學與分子生物學報，2005, 21(3), 376-383

(99) 陳建梁，關怡新，崔志芳，姚善涇，稀釋復性法體外復性重組牛凝血酶原-2包涵體，高校化學工程學報，2005, 19(2), 228-232

(100) 朱自強，關怡新，姚善涇，超臨界輔助霧化製備適用於氣溶膠給藥的藥物微粒，化工學報，2005, 56(2), 187-196

(101) 關怡新*，費崢崢，羅曼，姚善涇，小分子伴侶協助重組人γ-干擾素體外復性研究，生物化學與生物物理進展，2004, 31(10), 907-911

(102) 靳挺，關怡新，費崢崢，姚善涇，重組人γ-干擾素包涵體稀釋復性，化工學報，2004, 55(5), 770-774 ^[1] 

岑沛霖，關怡新，林建平，生物反應工程，高等教育出版社，2005年9月 ^[1] 

1、一種布氏乳桿菌及其應用，發明專利號：ZL 2016 1 0964912.X，授權日：2019年8月6日

2、布氏乳桿菌8-2N及在製備苯乳酸中的應用，發明專利號：ZL 2016 1 0906109.0，授權日：2019年6月14日

3、利用超臨界流體技術一步製備雙模式孔結構骨組織工程支架的方法，發明專利號：ZL 2016 1 0969582.3，授權日：2019年2月1日

4、混合式整體晶膠介質及其製備方法，發明專利號：ZL 2013 1 0152226.9，授權日：2015年8月5日

5、結合超臨界二氧化碳發泡技術製備具有雙孔結構組織工程支架的方法，發明專利號：ZL 2013 1 0203224.8，授權日：2015年4月15日

6、一種基於超臨界流體技術的黏膜附着性聚合物載藥貼膜製備方法，發明專利號：ZL 2013 1 0229174.0，授權日：2014年12月10日

7、一種基於超臨界流體技術的載藥傷口敷料製備方法，發明專利號：ZL 2013 1 0170625.8，授權日：2014年8月27日

8、一種超大孔晶膠微球及其製備方法，發明專利號：ZL 2011 1 0236064.8，授權日：2013年6月5日

9、一種基於超臨界流體技術的表面封閉載藥多孔聚合物微球製備方法，發明專利號：ZL 2012 1 0014825.X，授權日：2013年3月13日

10、粒狀聚(N-異丙基丙烯酰胺─丙烯酸鈉)共聚凝膠協助溶菌酶體外復性的方法，發明專利號：ZL 2008 1 0161657.0，授權日：2011年7月20日

11、結腸菌羣降解材料及其製備方法和用途，發明專利號：ZL 2007 1 0070812.3，授權日：2010年8月18日

12、6α-甲基強的松龍及其衍生物的生產方法，發明專利號：ZL 2005 1 0125689.1，授權日：2010年6月9日

13、水力空化增強超臨界輔助霧化製備微粒的系統及其方法，發明專利號：ZL 2007 1 0067262.X，授權日：2008年12月24日

14、1,4-孕二烯-16β-甲基-17α,21-二羥基物的製備方法，發明專利號：ZL 2006 1 0154573.5，授權日：2008年6月25日

15、常温加壓二氧化碳滅菌方法，發明專利號：ZL 2005 1 0061997.2，授權日：2007年7月11日

16、大孔型硫酸纖維素鈉－聚二甲基二烯丙基氯化銨生物微膠囊的製備方法，發明專利號：ZL 2004 1 0053010.8，授權日：2006年10月4日

17、温敏型聚N-異丙基丙烯酰胺凝膠協助溶菌酶復性的方法，發明專利號：ZL 2004 1 0073406.9，授權日：2006年8月2日

18 、由禽源性動物組織中製備膠原活性多肽的方法, 發明專利號：ZL 2004 1 0052758.6，授權日：2006年5月31日

19、內切β-1,4-葡聚糖酶的分離提純方法，發明專利號：ZL 2004 1 0054047.2，授權日：2006年4月26日

20、蚓激酶粗品中去除雜蛋白的方法，發明專利號：ZL 2004 1 0054043.4，授權日：2006年4月26日

21、用於温度誘導雙水相分配的金屬螯合親和配基及其製備方法，發明專利號：ZL 2004 1 0017753.X，授權日2006年3月15日

22、具有生物相容性的四組分微膠囊製備方法，發明專利號：ZL 03 1 29151.1，授權日2005年5月18日

23、均質型纖維素/鈦白粉複合微球及其製備方法，發明專利號：ZL 02 1 11928.7，授權日2004年9月1日

24、雙水相萃取法提取紅黴素的方法，發明專利號：ZL 98 1 04898.6，授權日2000年5月18日 ^[1] 

1、 纖維素高值化和功能化及生物工程中的應用探索，浙江省自然科學二等獎，2014

2、 擴張牀吸附介質研製及生物分離機制研究，浙江省科學技術二等獎，2010

3、 雙水相分配技術的基礎及工程研究，教育部科技進步一等獎，1999

4、生化反應工程課程建設與改革，浙江大學優秀教學成果二等獎，2008

1、 浙江省高等學校第三屆現代教學技能比賽優秀獎，2004

2、 浙江大學優質教學獎，2013

3、 浙江大學事業家庭兼顧型先進個人，2006

4、 浙江大學優秀研究生德育導師，2001、2004、2010、2012、2014

5、朱祖祥正大教育基金優秀教師獎，2001

6、 湯永謙、湯永年基金優秀中青年教師獎，1998、2008

7 、湯永謙、湯永年基金服務育人獎教金，2011、2013 ^[1]