張純

2019.02 - 至今 特聘副研究員，四川大學，華西藥學院

2017.12-2019.02 抗體下游工藝開發總監，康諾亞生物醫藥科技（成都）有限公司

2017.12-2018.01 副研究員，碩士生導師, 生化工程國家重點實驗室，中國科學院

2017.07-2017.12 副研究員，生化工程國家重點實驗室，中國科學院（北京）

2014.07-2017.07 助理研究員, 生化工程國家重點實驗室，中國科學院（北京）

2011.09-2014.07 四川大學，華西藥學院，微生物與生化藥學，理學博士 ^[1] 

《生物製藥學》

《藥用生物化學實驗》

《藥用分子生物學實驗》 ^[1] 

1.《生物技術製藥概論》（第4版，姚文兵主編），全國高等醫藥院校藥學類專業第五輪規劃教材，中國醫藥科技出版社，2019年12月，參編。

2.《藥用分子生物學》（餘蓉主編），化學工業出版社，2020年，參編。 ^[1] 

蛋白質/多肽藥物設計、發現與篩選。 ^[1] 

1.偏好性激活受體信號通路的蛋白/多肽藥物設計與篩選

2.藥用蛋白/多肽化學修飾與長效設計

3.基於人血清白蛋白的蛋白/多肽載體遞藥

4.抗體/多肽偶聯藥物及腫瘤靶向遞送

5.重組蛋白納米顆粒胞內/胞外自組裝與載藥

6.生物技術藥物高效下游技術研究與開發 ^[1] 

1.中國科協優秀中外青年交流計劃，中國科協技術協會，2020/01-2020/12, 負責人。

2.國家自然科學基金委員會，面上項目，81973226，雙靶點抗革蘭陰性菌新型抗菌肽-裂解酶蛋白的理性設計與機制研究。2020/01-2023/12, 主研。

3.四川大學引進人才科研啓動經費資助項目，YJ201926，2019-2022，負責人。

4.國家自然科學基金委員會，面上項目，21576267，病毒樣蛋白顆粒疫苗胞外自組裝機理及過程研究。2016/01-2019/12, 主研。

5.國家自然科學基金委員會，青年基金，21506228，分子輔助的蛋白質高濃度超濾膜復性的機理及應用研究。2016/01-2018/12, 主研。

6.北京市自然科學基金委員會，面上項目，2162041，融合蛋白中靶向肽RGD-4C定向成環策略及活性評價。2016/01-2018/12, 主研。

7.生化工程國家重點實驗室開放基金，開放基金，2015KF-05，靶向雙功能蛋白分子的設計及製備。2015/01-2017/12, 負責人。

8.中科院-諾和諾德專項研究經費，Novo Nordisk-CAS Research Fund，NNCAS-2014-2，Development of protein continuous refolding and purification technology。2015/01-2016/12, 主研。

9.中國醫學科科學院實驗動物研究所，橫向課題，492014103112801，重組多表位人腸道病毒71型抗原蛋白製備。2014/09-2015/06, 負責人。

10.教育部博士點基金，NO.20120181110036，雙功能Hv12p-rPA蛋白靶向溶栓設計及分子修飾。2013/01-2015/12, 主研。 ^[1] 

1.Xiangfeng Lv*, Chun Zhang*, Qingyun Shuaizhen, Rong Yu, Yongxiang Zheng, Design of integrin αvβ3 targeting self-assembled protein nanoparticles with RGD peptide, Biomedicine & Pharmacotherapy, Volume 128, 2020,

2.Chunyue Wang*, Chun Zhang*, Shuang Yin, Qi Wang, Rong Yu†, Yongdong Liu†, Zhiguo Su. Extending half-life of H-ferritin nanoparticle by fusing albumin binding domain for doxorubicin encapsulation. Biomacromolecules, 2018, 19 (3), pp 773–781.

3.Liping Liu*, Chun Zhang*, Zenglan Li, Chunyue Wang, Jingxiu Bi, Shuang Yin, Qi Wang, Rong Yu, Yong Dong Liu, and Zhiguo Su. Albumin binding domain fusing R/K-X-X-R/K sequence for enhancing tumor delivery of doxorubicin. Molecular Pharmaceutics, 2017, 14 (11), pp 3739–3749.

4.Chun Zhang*; Yu, Rong; Yongdong, Liu; Zhiguo, Su et al. Development of long-acting ciliary neurotrophic factor by site-specific conjugation with different-sized polyethylene glycols and transferrin. International Journal of Pharmaceutics. 2017; 529(1-2):275-284.

5.Yin, Shuang*; Zhang, Chun*; Li, Zenglan; Liu, Yongdong; Yu, Rong; Su, Zhiguo; Identification, characterization, and stabilization of the deamidation degradation of recombinant human tumor necrosis factor-α. Process Biochemistry. 2017; 53, pp216-223.

6.Xu Longfu*; Zhang, Chun*; Liu Yongdong, Zhiguo Su et al. Oxidized catechol-derived poly (ethylene glycol) for thiol-specific conjugation. Reactive and Functional Polymers, 117, 2017, P 97–105.

7.Zhang, Chun; Pu Jun, Yang Xiaolan, Feng Tao, Liu F, Wang D, Hu Xiaolei, Gao Ang, Liu Hongbo, Zhan Changguo, Liao Fei. Extracorporeal delivery of a therapeutic enzyme. Scientific Reports. 2016 Aug 1;6:30888. doi: 10.1038/srep30888.

8.Zhang, Chun; Liu, Yongdong; Feng, Cui; Wang, Qi; Shi, Hong; Zhao, Dawei; Yu, Rong; Su, Zhiguo, Loss of PEG chain in routine SDS-PAGE analysis of PEG-maleimide modified protein., Electrophoresis, 36(2), pp 371-374, 2015.

9.Zhang, Chun; Liu, Yongdong; Zhao, Dawei; Li, Xiunan; Yu, Rong; Su, Zhiguo, Facile purification of Escherichia coli expressed tag-free recombinant human tumor necrosis factor alpha from supernatant., Protein Expression Purification, 95, pp 195-203, 2014.

10.Zhang, Chun; Yang, Xiaolan; Gao, Ang; Hu, Xiaolei; Pu, Jun; Liu, Hongbo; Feng, Juan; Liao, Juan; Li, Yuanli; Liao, Fei, Comparison of modification of a bacterial uricase with N-hydroxysuccinimide esters of succinate and carbonate of monomethoxyl poly(ethylene glycol)., Biotechnology and Applied Biochemistry, 61(6), pp 683-690, 2014.

11.Zhang, Chun; Yang, Xiaolan; Yuan, Yong-hua; Pu, Jun; Liao, Fei, Site-specific PEGylation of therapeutic proteins via optimization of both accessible reactive amino acid residues and PEG derivatives., BioDrugs, 26(4), pp 209-215, 2012.

12.Gao, Ling*; Zhang, Chun*; Deng, Wutong; Su, Zhiguo; Yu, Rong, Construction, expression and refolding of a bifunctional fusion protein consisting of C-terminal 12-residue of hirudin-PA and reteplase., Protein Journal, 31(4), pp 328-336, 2012.

13.Zhang, Chun; Yang, Xiaolan; Feng, Juan; Yuan, Yonghua; Li, Xiang; Bu, Youquan; Xie, Yanling; Yuan, Huidong; Liao, Fei, Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus., Biosci Biotechnol Biochem, 74(6), pp 1298-1301, 2010.

14.Fangxia Guo, Yongdong Liu, Chun Zhang, Qi Wang, Lianyan Wang, Yuhui Gao, Jingxiu Bi, Heng Wang, and Zhiguo Su, Prompt and Robust Humoral Immunity Elicited by a Conjugated Chimeric Malaria Antigen with a Truncated Flagellin. Bioconjugate Chemistry, 2018 29(3):761-770.

15.Zenglan Li, Chun Zhang, Yan Zhang, Yongdong Liu, Xiunan Li, Guanghui Ma, Jian Luo, Zhiguo Su, Prevention of aggregate formation through mechanism analysis in refolding of recombinant pertactin from Escherichia coli, Process Biochemistry, 2017, 62:80-90.

16.Wang Q, Zhang Chun, Liu LP, Li ZP, Guo FX, Li XN, Luo J, Zhao DW, Liu YD, Su ZG. High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency. Journal of Biotechnology, 2017 20;254:34-42.

17.Wang Q, Liu YD, Zhang Chun, Guo FX, Feng C, Li XN, Shi H, Su ZG. High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration. Protein Expression and Purification, 2017, 133: 152-59.

18.Longfu Xu, Chun Zhang, Liping Liu, Yao Zhang, Qi Wang, Jian Wang, Yongdong Liu, Zhiguo Su, Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain, Protein Expression and Purification, Vol 139, 2017, Pages 14-20.

19.Xue L, Liu JN, Wang Q, Zhang Chun, Xu LF, Luo J, Wang J, Qin C, Liu YD, Su ZG. Purification and assembling a fused capsid protein as an enterovirus 71 vaccine candidate from inclusion bodies to pentamer-based nanoparticles. Biochemical Engineering Journal, 2017, 117: 139–146

20.Chen Y, Wang Q, Zhang Chun, Li X, Gao Q, Dong C, Liu Y, Su Z. Improving the refolding efficiency for proinsulin aspart inclusion body with optimized buffer compositions. Protein Expr Purif. 2016;122:1-7.

21.Zhao DW, Liu YD, Zhang GF, Zhang Chun, Li XN, Wang QQ, Shi H, Su ZG. Interaction of arginine with protein during refolding process probed by amide H/D exchange mass spectrometry and isothermal titration calorimetry. Biochimica et Biophysica Acta. 2015, 1854: 39–45.

22.Gao A, Yang X, Zhang Chun, Long G, Pu J, Yuan Y, Liu H, Li Y, Liao F. Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives. Chem Cent J. 2012, 23;6(1):142.

23.Feng J, Chen Y, Pu J, Yang X, Zhang Chun, Zhu S, Zhao Y, Yuan Y, Yuan H, Liao F. An improved malachite green assay of phosphate: mechanism and application. Anal Biochem. 2011 Feb 1;409(1):144-9.

24.Yang X, Xie Y, Pu J, Zhao H, Liao J, Yuan Y, Zhu S, Long G, Zhang Chun, Yuan H, Chen Y, Liao F. Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model. BMC Biotechnol. 2011 May 5;11:44.

25.Feng J, Li X, Yang X, Zhang Chun, Yuan Y, Pu J, Zhao Y, Xie Y, Yuan H, Bu Y, Liao F. A new practical system for evaluating the pharmacological properties of uricase as a potential drug for hyperuricemia. Arch Pharm Res. 2010 Nov;33(11):1761-9.

26.Zhao Y, Yang X, Li X, Bu Y, Deng P, Zhang Chun, Feng J, Xie Y, Zhu S, Yuan H, Yu M, Liao F. Reversible inactivation of an intracellular uricase from Bacillus fastidiosus via dissociation of homotetramer into homodimers in solutions of low ionic strength. Biosci Biotechnol Biochem. 2009 Sep;73(9):2141-4.

27.遲勝男，李增蘭，張純^#，殷爽，馮翠，王祺，劉永東^#，蘇志國；聚乙二醇20k修飾與轉鐵蛋白偶聯睫狀神經營養因子的生物活性對比研究。中國生物工程雜誌； 2017 (12):59-66.

28.劉立平，張純^#，殷爽，王祺，張耀，餘蓉，劉永東^#，蘇志國；白蛋白結合肽-多柔比星耦合物的設計、製備、表徵及初步評價。中國生物工程雜誌；2017, 37（4）68-75.

29.薛玲，張耀，張純，王琪，劉江寧，秦川，劉永東，蘇志國；親和純化EV71融合多表位蛋白抗原及其自組裝為病毒樣蛋白顆粒。中國生物工程雜誌，2016，36 (7) :34-40

30.殷爽，馮翠，張純，王祺，王健，餘蓉，劉永東，蘇志國；轉鐵蛋白-PEG-睫狀神經營養因子的製備及其生物活性評價。中國生物工程雜誌，2016,36（4）：43-49

31.馮翠，王祺，張純，秦培勇，鄭秀玉，王健，劉永東，蘇志國；PEG定點修飾重組人睫狀神經營養因子及其生物活性評價。中國生物工程雜誌，2015,35（5）：15-21

32.馮翠 趙大偉 張純 王健 秦培勇 劉永東 蘇志國；一種重組人睫狀神經營養因子突變體的分離純化及結構鑑定。中國生物工程雜誌，2013，10：21-27

33.甘志勇，張純，馮娟，楊曉蘭，袁擁華，廖飛，尿酸酶在用氧嗪酸建立高尿酸血癥模型大鼠體內的降尿酸作用，重慶醫科大學學報，2010.01.01，35（10）：1454~1456

34.Zhang, Chun; Liu Yongdong; Yu Rong，Ma Guanghui, Su Zhiguo; Instability of Thioether Bond Misleads the Observation of PEG-MAL Modified Protein in Routine SDS-PAGE Analysis，Biochemical and Molecular Engineering XVIII, 2013.06.16-20. Beijing, China. (Poster)

35.Zhang, Chun; Liu Yongdong，Feng Cui，Wang Qi，Su Zhiguo, Developing Long-Acting Ciliary Neurotrophic Factor by Conjugation with Different Size of PEGs or Transferrin, 21th Symposium Series of Young Asian Biochemical Engineers’ Community (YABEC 2015), 2015.10.14-16. Chuncheon, Korea. (Poster) ^[1] 

1.ZL 201410437913.X. 劉永東，蘇志國，王祺，張純。重組人干擾素β1b高壓復性及組合層析製備方法。

2.201710859565.9. 張純，劉永東，蘇志國。一種提高離子交換層析內毒素去除效率的方法及其用途。

3.201710875115.9. 劉永東，張純，蘇志國。一種納米載體蛋白及其製備方法和應用。

4.201710184644.4. 劉永東，徐龍福，張純，蘇志國。一種長效重組睫狀神經營養因子及其製備方法用途。

5.201610543500.9. 劉永東，王祺，蘇志國，張純。一種以鐵蛋白為載體的抗腫瘤藥物及其製備方法。

6.201610616651.2. 劉永東，徐龍福，蘇志國，張純，王祺。一種含有鄰苯二醌類官能團的聚乙二醇修飾劑及其製備方法和用途。

7. 201610899293.0. 劉永東，張純，蘇志國。一種腫瘤靶向多肽-藥物偶聯衍生物、其製備方法及應用。 ^[1]