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覃重軍

鎖定
覃重軍，1965年2月生，籍貫湖南省邵陽市 [1]  ，中科院分子植物科學卓越創新中心研究員，中科院合成生物學重點實驗室主任，國家傑出青年基金獲得者。從事合成微生物、藥物生物製造等方面的研究。在國際上首次人工創建單條染色體的真核細胞釀酒酵母。與企業合作利用基因組工程構建了抗寄生蟲藥物多拉菌素等工業生產菌株，打破了國外壟斷。 [2] 
中文名
覃重軍
外文名
qin zhong jun
國    籍
中國
民    族
漢族
出生日期
1965年
畢業院校
武漢大學
主要成就
創建出了國際首例人造單染色體真核細胞
職    稱
研究員
性    別
男
學位/學歷
博士

目錄

  1. 1 個人簡歷
  2. 2 研究方向
  3. 3 研究工作
  1. 4 學術成就
  2. 5 人物評價
  3. 6 自我評價
  1. 7 發表論文

覃重軍個人簡歷

覃重軍 覃重軍 [3]
覃重軍，1965年生 [2]  ，1987年畢業於武漢大學生物系微生物專業，獲學士學位。 1992年畢業於華中農業大學微生物系，獲博士學位。 1994年被破格提拔為副教授。主持國家自然科學青年基金和湖北省自然科學基金各一項。 1995年至2000年在美國斯坦福大學基因工程創始人之一Stanley N. Cohen教授實驗室作博士後, 從事鏈黴菌線型質粒的複製、重組、修復、 接合轉移、端粒基因、異常DNA結構等方面的研究。 2001年通過“引進國外傑出人才計劃”答辯, 被中國科學院上海植物生理研究所聘為研究員、博士生導師。 2002年任微生物次生代謝分子調控研究開放實驗室主任。 2003年獲國家傑出青年科學基金資助。 2001年回國後主持國家“863”項目和國家自然科學基金共4項，主持上海市、科學院、所基金共5項。
1994年，覃重軍確立了自己的5個科學夢想：要在重大基礎研究、原創技術、產業化、解決人類疾病、新理論上有所突破。 [2] 
2013年5月8日，覃重軍瞄準了真核單細胞生物的模式材料——釀酒酵母，花了5年時間，用基因編輯的方法，將釀酒酵母中16條天然染色體合成為1條，創建出了國際首例人造單染色體真核細胞，實現“人造生命”里程碑式的重大突破。該成果於2018年8月2日在線發表在《自然》雜誌上。 [1] 

覃重軍研究方向

分子微生物學和分子微生物組學

覃重軍研究工作

從事分子微生物學、分子微生物組學和微生物藥物代謝工程的研究，主要內容包括：（1）放線菌線型染色體和線型質粒的生物學功能。（2）中草藥植物內生放線菌。（3）放線菌基因組工程。（4）微生物藥物工業生產菌種的遺傳改良。 [3] 

覃重軍學術成就

博士畢業論文發表在國際知名專業期刊《細菌學雜誌》上，這也是華中農大在該本雜誌上發表的第一篇學術文章。 [2] 
兩年前，覃重軍帶領團隊將釀酒酵母中16條天然染色體合成為1條，創建出了國際首例人造單染色體真核細胞。這一成果在線發表在《自然》雜誌上，被業界認為是合成生物學具有里程碑意義的重大突破。 [2] 
帶領團隊首次在粘細菌中發現質粒並建立遺傳操作系統，全世界有20多個實驗室來函索取質粒載體；對大腸桿菌的基因組進行了“刪減、重組、優化、合成”等操作，做了大量有益的探索。他的團隊與國內製藥企業合作改良了抗寄生蟲藥物多拉菌素，打破了國外在該領域的壟斷，藥品銷售額超過億元。 [1] 

覃重軍人物評價

我在你身上感受到了對科學的熱愛以及想要取得突破的強烈願望。（基因工程的創始人之一、美國斯坦福大學的斯坦利·科恩教授） [2] 

覃重軍自我評價

覃重軍在博士論文的後記中寫道：“近1300頁的實驗記錄中，失敗何止百次！然而，沒有一次失敗能夠真正打垮我，為什麼？照亮我前進道路並不斷給我勇氣去正視失敗的，是對科學的熱愛，對揭示生命世界奧秘的嚮往以及科學發現給我帶來的純真快樂……對我而言，科學是一條無窮無盡的探索之路，也許一生都會這樣幹下去。” [1-2] 

覃重軍發表論文

1. Yangyang Shao, Ning Lu, Zhenfang Wu, Chen Cai, Shanshan Wang, Ling-Li Zhang, Fan Zhou, Shijun Xiao, Lin Liu, Xiaofei Zeng, Huajun Zheng, Chen Yang, Zhihu Zhao, Guoping Zhao*, Jinqiu Zhou*, Xiaoli Xue* and Zhongjun Qin* (2018) Creating a functional single chromosome yeast. Nature (article). 560:331–335.
2. Yangyang Shao, Ning Lu, Chen Cai, Fan Zhou, Shanshan Wang, Zhihu Zhao*, Guoping Zhao*, Jinqiu Zhou*, Xiaoli Xue* and Zhongjun Qin* (2018). A single circular chromosome yeast. Cell Research. 10.1038/s41422-018-0110-y.
3. Yangyang Shao, Ning Lu, Zhongjun Qin* and Xiaoli Xue* (2018). CRISPR-Cas9 facilitated multiple-chromosome fusion in Saccharomyces cerevisiae. ACS Synthetic Biology. 10.1021/acssynbio.8b00397.
4. Yangyang Shao, Ning Lu, Xiaoli Xue* and Zhongjun Qin* (2018). CRISPR-Cas9 facilitated multiple-chromosome fusion in Saccharomyces cerevisiae. Nature Protocol. Invited.
5. Jianting Zhou, Ronghai Wu, Xiaoli Xue* and Zhongjun Qin* (2016) CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA. Nucleic Acids Research. 4(14):e124.
6. Xiaoli Xue*, Tao Wang, Peng Jiang, Yangyang Shao, Min Zhou, Li Zhong, Ronghai Wu, Jianting Zhou, Haiyang Xia, Guoping Zhao and Zhongjun Qin* (2015) MEGA (Multiple Essential Genes Assembling) deletion and replacement method for genome reduction in Escherichia coli. ACS Synthetic Biology. 4(6):700–706.
7. Jian Chen, Haiyang Xia*, Fujun Dang, Qingyu Xu, Wenjun Li and Zhongjun Qin* (2015) Characterization of the chromosomal integration of Saccharopolyspora plasmid pCM32 and its application to improve production of spinosyn in Saccharopolyspora spinosa. Applied Microbiology and Biotechnology. 99:10141–10149.
8. Yalan Chen, Huarong Tan and Zhongjun Qin* (2013) Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted-repeats in Streptomyces plasmid pSV1. FEMS Microbiology Letters. 349: 144–152.
9. Shiyuan Peng, Ana Zeng, Li Zhong, Ran Zhang, Min Zhou, Qiuxiang Cheng, Liqian Zhao, Tao Wang, Huarong Tan and Zhongjun Qin* (2013) Three functional replication origins of the linear and artificially-circularized plasmid SCP1 of Streptomyces coelicolor. Microbiology. 159: 2127–2140.
10. Ran Zhang, Haiyang Xia, Qingyu Xu, Fujun Dang and Zhongjun Qin* (2013) Recombinational cloning of the antibiotic biosynthetic gene clusters in linear plasmid SCP1 of Streptomyces coelicolor A3(2). FEMS Microbiology Letters. 345: 39–48.
11. Pengfei Xie, Ana Zeng and Zhongjun Qin* (2013) A putative transglycosylase encoded by SCO4132 influences morphological differentiation and actinorhodin production in Streptomyces coelicolor. Acta Biochimica et Biophysica Sinica. 45(4):296–302.
12. Tao Wang, Zhenhua Chen, Qiuxiang Cheng, Min Zhou, Xinli Tian, Pengfei Xie, Li Zhong, Meijuan Shen and Zhongjun Qin* (2012) Characterization of replication and conjugation of plasmid pWTY27 from a widely distributed Streptomyces species. BMC Microbiology. 12:253.
13. Zhenhua Chen, Li Zhong, Meijuan Shen, Ping Fang and Zhongjun Qin* (2012) Characterization of Streptomyces plasmid-phage pFP4 and its evolutionary implications. Plasmid. 68:170–178.
14. Min Zhou, Xinyun Jing, Pengfei Xie, Weihua Chen, Tao Wang, Haiyang Xia and Zhongjun Qin* (2012) Sequential deletion of all the PKS and NRPS biosynthetic gene clusters and a 900-kb subtelomeric sequence of the linear chromosome of Streptomyces coelicolor. FEMS Microbiology Letters. 333(2):169–179.
15. Yun Fan, Yumei Dai, Qiuxiang Cheng, Guangjun Zhang, Dongshu Zhang, Ping Fang*, Hang Wu, Linquan Bai*, Zixin Deng and Zhongjun Qin* (2012) A self-ligation method for PCR-sequencing the telomeres of Streptomyces and Mycobacterium linear replicons. Journal of Microbiological Methods. 90:105–107.
16. Weihua Chen and Zhongjun Qin* (2011) Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters. BMC Microbiology. 11(1):243.
17. Ana Zeng, Tao Wang, Haiyang Xia, Shiyuan Peng, Weihua Chen, Chenglin Jiang, Lihua Xu, Li Zhong, Meijuan Shen and Zhongjun Qin* (2011) Development of a vector and host system and characterization of replication of plasmid pSQ10 in moderately halophilic Nocardiopsis. Acta Biochimica et Biophysica Sinica. 43(9):738–743.
18. Peng Guo, Qiuxiang Cheng, Pengfei Xie, Yun Fan, Weihong Jiang and Zhongjun Qin* (2011) Characterization of the multiple CRISPR loci on Streptomyces linear plasmid pSHK1. Acta Biochimica et Biophysica Sinica. 43(8):630–639.
19. Li Zhong, Qiuxiang Cheng, Xinli Tian, Liqian Zhao and Zhongjun Qin* (2010) Characterization of the replication, transfer and plasmid/lytic phage cycle of the Streptomyces plasmid-phage pZL12. Journal of Bacteriology. 192:3747–3754.
20. Ran Zhang, Shiyuan Peng and Zhongjun Qin* (2010) Two internal origins of replication on Streptomyces linear plasmid pFRL1. Applied and Environmental Microbiology. 76(17):5676–5683.
21. Zhiqun Lu, Pengfei Xie and Zhongjun Qin* (2010) Promotion of markerless deletion of the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor. Acta Biochimica et Biophysica Sinica. 42(10):717–721.
22. Pengfei Xie, Ana Zeng and Zhongjun Qin* (2009) cmdABCDEF, a cluster of genes encoding membrane proteins for differentiation and antibiotic production in Streptoymyces coelicolor A3(2). BMC Microbiology. 9:157.
23. Yongqiang Tian, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2009) In vivo conjugation-coupled recombinational cloning of a Streptomyces lividans chromosomal telomeric DNA using a linear plasmid. Biotechnology Letter. 31(8):1253–1258.
24. Ran Zhang, Haiyang Xia, Peng Guo and Zhongjun Qin* (2009) Variation in the replication loci of Streptomyces linear plasmids. FEMS Microbiology Letters. 290:209–216.
25. Ran Zhang, Ana Zeng, Ping Fang and Zhongjun Qin* (2008) Characterization of the replication and conjugation loci of Streptomyces circular plasmids pFP11 and pFP1 and their ability to propagate in linear mode with artificially attached telomeres. Applied and Environmental Microbiology. 74(11):3368–3376.
26. Jingyi Zhao, Li Zhong, Meijuan Shen, Zhijie Xia, Qiuxiang Cheng, Xia Sun, Guoping Zhao, Yuezhong Li* and Zhongjun Qin* (2008) Discovery of an autonomously-replicating plasmid pMF1 from Myxococcus fulvus and the development of a gene cloning system in Myxococcus Xanthus. Applied and Environmental Microbiology. 74(7):1980–1987.
27. Mingxuan Xu, Yingmin Zhu, Ran Zhang, Meijuan Shen, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2006) Characterization of the genetic components of Streptomyces lividans linear plasmid SLP2 for replication in circular and linear modes. Journal of Bacteriology.188:6851–6857.
28. Ran Zhang, YongYang, Ping Fang, Chenglin Jiang, Lihua Xu, Yingmin Zhu, Meijuan Shen, Haiyang Xia, Jianfu Zhao, Tongbing Chen and Zhongjun Qin* (2006) Diversity of telomere palindromic sequences and replication genes among Streptomyces linear plasmids. Applied and Environmental Microbiology. 72(9):5728–5733.
29. Meijuan Shen, Ping Fang, Deqiang Xu, Yalei Zhang, Weihuan Cao, Yingmin Zhu, JianfuZhao and Zhongjun Qin* (2006) Replication and Inheritance of Nocardia Plasmid pC1. FEMS Microbiology Letters. 261:47–52.
30. Haiyang Xia, Yongqiang Tian, Ran Zhang, Kaichun Lin and Zhongjun Qin* (2006) Characterization of Nocardia Plasmid pXT107. Acta Biochimica et Biophysica Sinica. 38(9):620–624.
31. Mingxuan Xu, Yingmin Zhu, Meijuan Shen, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2006) Characterization of the essential genetic components for conjugal transfer of Streptomyces lividans linear plasmid SLP2. Progress in Biochemistry and Biophysics. 33(10):1–8.
32. Yongqiang Tian, Pei Hao, G.uoping Zhao* and Zhongjun Qin* (2005) Cloning and characterization of the chromosomal replication origin region of Amycolatopsis mediterranei U32. Biochemical and Biophysical Research Communications. 333(1):14-20.
33. Zhongjun Qin, Meijuan Shen and Stanley N. Cohen* (2003) Identification and characterization of a pSLA2 plasmids locus required for linear DNA replication and circular plasmids stable inheritance in Streptomyces lividans. Journal of Bacteriology. 185 (22):6575–6582.
34. Zhongjun Qin and Stanley N. Cohen* (2002) Survival mechanisms for Streptomyces linear replicons after telomere damage. Molecular Microbiology. 45(3):785–794.
35. Zhongjun Qin and Stanley N. Cohen* (2000) Long palindrome formed in Streptomyces by nonrecombinational intra-strand annealing. Genes&Development. 14:1789–1796.
36. Zhongjun Qin and Stanley N. Cohen* (1998) Replication at the telomeres of Streptomyces linear plasmid pSLA2. Molecular Microbiology. 28:893–903.
37. Zhongjun Qin, Kaiman Peng, Xiufen Zhou, Rongfang Liang, Qi Zhou, Huakui Chen, David A. Hopwood, Tobias Kieser and Zixin Deng* (1994) Development of a gene cloning system for Streptomyces hygroscopicus subsp. yingchenggenesis, a producer of the three useful antifungal compounds, by elimination of three barriers to DNA transfer. Journal of Bacteriology. 176:2090–2095. [4] 
參考資料