時光

1.表觀遺傳修飾和基因表達調控方向研究；

2.胚胎幹細胞維持自我更新和分化的表觀遺傳學研究。 ^[1] 

生物化學I，遺傳學實驗，幹細胞和細胞分化，衰老與抗衰老 ^[1] 

應用生物化學技術剖析組蛋白去甲基化酶PHF8的表觀遺傳的作用位點，能去除H3K9me1/2和H3K4me1/2/3位點的甲基化修飾。在X染色體連鎖的精神障礙（X-linked Mental Retardation，XLMR）疾病中存在PHF8基因的酶活突變體，通過體外小鼠神經細胞分化模型揭示了其酶活突變體抑制小鼠幹細胞向神經細胞分化（Cell Research，2010）；通過表觀遺傳機制探究了XLMR的致病機理；又探究了同家族蛋白PHF2與組蛋白甲基化酶SUV39H1相互作用，協同抑制核糖體DNA的轉錄（Journal of Biological Chemistry，2014）；挖掘出了SUMO修飾抑制整體基因轉錄的新機制，即通過蛋白激酶CDK9的SUMO修飾抑制其激酶活性，從而降低RNA聚合酶II CTD結構域的2號絲氨酸的磷酸化水平，同時探究了MYC激活基因轉錄和SUMO修飾抑制基因轉錄之間的關聯性（Cell Research，2018）。 ^[1] 

1.廣東省自然科學基金自由申請項目，2017A030313093，Bend4基因在小鼠胚胎幹細胞定向分化成生殖祖細胞過程中的功能研究，2017.05-2020.05，10萬，在研，主持。

2.國家自然科學基金面上項目，31671540，Bend5調控胚胎幹細胞分化為原始生殖細胞的分子機制研究，2017.01-2020.12，65萬，在研，參加。

3.國家自然科學基金青年項目，31301082、CTCF對體細胞重編程不同階段的細胞染色質組織調控的機制研究，2014/01-2016/12，23萬元，已結題，主持。 ^[1] 

(1) Fang Yu#,Guang Shi#, Shimeng Chen, Jiwei Chen, Shwu-Yuan Wu, Zhiqiang Wang, nansong xia, Yunhao Zhai, Zhenxing Wang, Yu Peng, Dong Wang, James X. Du, Lujian Liao, Sheng-Zhong Duan, Tieliu Shi, Jinke Cheng, Cheng-Ming Chiang, Jiwen Li, Jiemin Wong. SUMO Suppresses and MYC Amplifies Transcription Globally by Regulating CDK9 Sumoylation.Cell Research, 2018.Feb 11(Accepted).

(2) Yang Hu#,Guang Shi#*, Laichen Zhang, Feng Li, Yuanling Jiang, Shuai Jiang, Wenbin Ma, Yong Zhao, Zhou Songyang, Junjiu Huang*. Switch telomerase to ALT mechanism by inducing telomeric DNA damages and dysfunction of ATRX and DAXX.Scientific Report.2016.Aug 31; 6:32280.

(3)Guang Shi#,Meng Wu#,Lan Fang,Fang Yu,Shimeng Cheng,Jiwen Li,James X. Du,Jiemin Wong*. PHD Finger Protein 2 (PHF2) Represses Ribosomal RNA Gene Transcription by Antagonizing PHD Finger Protein 8 (PHF8) and Recruiting Methyltransferase SUV39H1. Journal of Biological Chemistry. 2014. 289(43): 29691-700

(4) Jihui Qiu#,Guang Shi#, Yuanhui Jia#, Jing Li, Meng Wu, Jiwen Li, Shuo Dong, Jiemin Wong*, The X-linked mental retardation gene PHF8 is a histone demethylase involved in neuronal differentiation. Cell Research, 2010. 20(8): 908-918.

(5)Kaimeng Huang#, Xiaobai Zhang#, Jiejun Shi#, Mingze Yao, Jiannan Lin, Jiao Li, He Liu, Huanhuan Li,Guang Shi, Zhibin Wang, Biliang Zhang, Jiekai Chen, Guangjin Pan, Cizhong, Jiang, Duanqing Pei*, Hongjie Yao*. Dynamically reorganized chromatin is the key for the reprogramming of somatic cells to pluripotent cells. Scientific Reports. 2015, 5:17691.

(6)Sijie Zhang#, Zhenhua Luo,Guang Shi, Dan Liu, Zhou Songyang*,JunjiuHuang*.TPP1 as a versatile player at the ends of chromosomes.Front. Biol2014.

(7)Junjiang Fu#, Jun Jiang, Jiwen Li, Shanshan Wang, Guang Shi, Qin Feng, Eileen White, Jun Qin, Jiemin Wong*. Deleted in breast cancer 1, a novel androgen receptor (AR) coactivator that promotes AR DNA-binding activity. Journal of Biological Chemistry, 2009. 284(11): 6832-6840. ^[1]