李爽

2001.9-2006.7 清華大學化學工程系 生物化工研究所 攻讀博士

1997.7-2001.7 清華大學化學工程系 本科

2006.8-2007.6 廣藥集團廣州拜迪生物醫藥有限公司，擔任科研開發部部長

2007.8-至今 華南理工大學生物科學與工程學院

本科課程：《遺傳學》《基因診斷與基因治療》《細胞生物學實驗》《生物化學實驗》

研究生課程：《科學實驗設計與實驗技能培訓》

關於改善甘油脱水酶機理性失活的研究，國家自然科學基金青年科學基金項目

以發展合成生物學的方法為主要研究方向，建立合成生物學的關鍵技術平台。針對資源環境與人類健康等方面的需求，在分子和細胞水平上開展研究，着重關注若干重要化學品的生物製造。

（1） 利用合成生物學的工具，指導改造或合成新型的或改良的生物學體系（細胞工廠），以生產生物能源燃料及生物醫藥化學品。

（2） 通過代謝途徑(網絡)不斷的改造和優化，系統提高目標產物產量，或降低毒害中間產物的產生與積累。

（3） 利用蛋白/酶改造技術，有針對性的改造細胞工廠內關鍵蛋白/酶，提升反應效率。

[1] 發明專利：Li S，Lai Z, Wang J.. A Thermophilic ananrobic Bacillus and a method for producing ethanol by using the same. 國際專利11201701615R，授權時間：2017.9.26

[2] 發明專利：李爽，朱牧孜，黃雄亮，王菊芳. 一種嗜熱厭氧桿菌及其在生產乳酸中的應用（201510315114.X），授權時間：2019.1.29

[3] 發明專利：李爽, 賴志城, 王菊芳. 一種嗜熱厭氧桿菌及利用其生產乙醇的方法. 201410453513.8，授權時間：2017.2.1

[4] 發明專利：李爽, 楊曉鋒, 林章凜, 王菊芳, 王小寧. 基於自聚集短肽誘導的固定化腈水解酶及製備方法和應用. 201410019182.7，授權時間：2016.3.2

[5] 實用新型：李爽, 朱晁誼, 歐陽小丹. 一種連續生產乙醇的裝置. 201721657272.4，授權時間：2018.9.7

[6] 軟件著作權：李爽，馬浩，倪夢姣，卓敏. 基於Android智能手機的水樣中重金屬離子的半定量檢測軟件, 2019SR0090522, 登記時間：2018.6.1

1. Zhu M, Zhang L, Yang F, Cha Y, Li S*, Zhuo M, Huang S, Li J. A recombinant β-mannanase from Thermoanaerobacterium aotearoense SCUT27: Biochemical characterization and its thermostability improvement. J Agr Food Chem 2020, 68: 818-825.

2. Chen H, Zhu C, Zhu M, Xiong J, Ma H, Zhuo M, and Li S*. High production of valencene in Saccharomyces cerevisiae through metabolic engineering. Microb Cell Fact. 2019, 18:195.

3. Yuan P, Dong J, Zhao W, Zhuo M, Li S*, Huang S, and Li J. Qualitative and quantitative assessment of genotoxins using SRRz lysis reporter under the control of a newly designed SOS responsive promoter in Escherichia coli. RSC Advances. 2019, 9:35662-35670.

4. Huang S, Huang X, Li S, Zhu M, Zhuo M. MHC class I allele diversity in cynomolgus macaques of Vietnamese origin. PeerJ. 2019, 7:e7941.

5. Ye F, Liu S, Yang Y, Zhao T, Li S, Zhou T, et al. Identification of the major metabolites of (R)-salbutamol in human urine, plasma and feces using ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. J Sep Sci. 2019, 42:3200-8.

6. Ouyang, X,Cha Y, Li W, Zhu C, Zhu M, Li S*, Zhuo M, Huang S and Li J. Stepwise engineering of Saccharomyces cerevisiae to produce (+)-valencene and its related sesquiterpenes. RSC Advances, 2019, 9:30171-30181.

7. Xu T, Huang X, Li Z, Ki Lin C S, Li S*, Enhanced purification efficiency and thermal tolerance of Thermoanaerobacterium aotearoense β-Xylosidase through aggregation triggered by short peptides. J Agr Food Chem 2018, 66: 4182-4188.

8. Xu H, Tong N, Huang S, Hayat W, Fazal S, Li J, Li S, Yan J, Zhang Y, Simultaneous autotrophic removal of sulphate and nitrate at different voltages in a bioelectrochemical reactor (BER): Evaluation of degradation efficiency and characterization of microbial communities. Bioresour Technol 2018, 265:340-348.

9. Xu H, Tong N, Huang S, Zhou S, Li S, Li J, Zhang Y, Degradation of 2,4,6-trichlorophenol and determination of bacterial community structure by micro-electrical stimulation with or without external organic carbon source. Bioresour Technol 2018, 263:266-272.

10. Du C, Abdullah JJ, Greetham D, Fu D, Yu M, Ren L, Li S, Lu D. Valorization of food waste into biofertiliser and its field application. J Clean Prod 2018, 187:273-284.

11. Zhu M, Fan W, Cha Y, Yang X, Lai Z, Li S*, Wang, X. Dynamic cell responses in Thermoanaerobacterium sp. under hyperosmotic stress. Sci Rep, 2017, 7:10088.

12. Yang X, Zhu M, Huang X, Lin CSK, Wang J, Li S*, Valorisation of mixed bakery waste in non-sterilized fermentation for l-lactic acid production by an evolved Thermoanaerobacterium sp. strain. Bioresource Technol, 2015, 198:47-54.

13. Zhu M, Lu Y, Wang J, Li S*, Wang X, Carbon catabolite repression and the related genes of ccpA, ptsH and hprK in Thermoanaerobacterium aotearoense. PLoS ONE, 2015, 10:e0142121. (JCR Q1, IF2.776)

14. Huang X, Li Z, Du C, Wang J, Li S*, Improved expression and characterization of a multi-domain xylanase from Thermoanaerobacterium aotearoense SCUT27 in Bacillus subtilis. J Arg Food Chem, 2015, 63:6430-6439.

15. Yang F, Yang X, Li Z, Du C, Wang J, Li S*, Overexpression and characterization of a glucose-tolerant β-glucosidase from T. aotearoense with high specific activity for cellobiose. Appl Microbiol Biotechnol, 2015, 99:8903-8915.

16. Yang X, Huang A, Peng J, Wang J, Wang X, Lin Z*, Li S*, Self-assembly amphipathic peptides induce active enzyme aggregation that dramatically increases the operational stability of nitrilase, RSC Advances, 2014, 4: 60675-60684.

17. Lai Z, Zhu M, Yang X, Wang J, Li S* Optimization of key factors affecting hydrogen production from sugarcane bagasse by a thermophilic anaerobic pure culture. Biotechnol Biofuels 2014, 7:119. (JCR Q1, IF3.6.22)

18. Ai H, Zhang J, Yang M, Yu P, Li S, Zhu M, Dong H, Wang S, Wang J: Draft Genome Sequence of an Anaerobic, Thermophilic Bacterium, Thermoanaerobacterium aotearoense SCUT27, Isolated from a Hot Spring in China. Genome Announc 2014, 2.

19. Yang X, Lai Z, Lai C, Zhu M, Li S*, Wang J*, Wang X: Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoense with broad substrate specificity. Biotechnol Biofuels 2013, 6:124.

20. Xu W, Chooi YH, Choi JW, Li S, Vederas JC, Da Silva NA, Tang Y: LovG: the thioesterase required for dihydromonacolin L release and lovastatin nonaketide synthase turnover in lovastatin biosynthesis. Angew Chem Int Ed Engl 2013, 52:6472-6475.

21. Li L, Ai H, Zhang S, Li S, Liang Z, Wu ZQ, Yang ST, Wang JF: Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum. Bioresour Technology 2013, 143:397-404.

22. Liang ZX, Li L, Li S, Cai YH, Yang ST, Wang JF: Enhanced propionic acid production from Jerusalem artichoke hydrolysate by immobilized Propionibacterium acidipropionici in a fibrous-bed bioreactor. Bioprocess Biosyst Eng 2012, 35:915-921. IF=1.82

23. Li S, Yang X, Yang S, Zhu M, Wang X: Technology prospecting on enzymes: Application, marketing and engineering. Comput Struct Biotechnol J 2012, 2:e201209017.

24. Cai YH, Liang ZX, Li S, Zhu MJ, Wu ZQ, Yang ST, Wang JF: Bioethanol from fermentation of cassava pulp in a fibrous-bed bioreactor using immobilized Δldh, a genetically engineered Thermoanaerobacterium aotearoense. Biotechnol Bioproc Eng 2012, 17:1270-1277.

25. Cai Y, Lai C, Li S, Liang Z, Zhu M, Liang S, Wang J: Disruption of lactate dehydrogenase through homologous recombination to improve bioethanol production in Thermoanaerobacterium aotearoense. Enzyme Microb Tech 2011, 48:155-161.

26.Li S, Lai C, Cai Y, Yang X, Yang S, Zhu M, Wang J, Wang X: High efficiency hydrogen production from glucose/xylose by the ldh-deleted Thermoanaerobacterium strain. Bioresour Technol 2010, 101:8718-8724.