謝勝松

教育經歷

2002.09 - 2006.06， 華中農業大學，動物科學專業畢業，農業學士

2006.09 - 2009.06， 華中農業大學，動物遺傳育種與繁殖專業畢業，農學碩士

2009.09 - 2013.03， 中科院上海生命科學研究院生物化學與細胞生物學研究所，生物化學與分子生物學專業畢業，理學博士

工作經歷

2013.04-2014.11， 中科院生物化學與細胞生物學研究所、助理研究員

2014.12-至今， 華中農業大學、副教授，碩士研究生導師 ^[1] 

小分子非編碼RNA在豬的雄性生殖系統和幹細胞乾性維持中的作用機理，基因組編輯技術優化，軟件與數據庫開發 ^[1]  。

1.Xie S, Feng Y, Shen Y, Qin H, Zhu M, Zhao S. Cloning of Porcine miRNA let-7b and let-7c by Two Methods. Journal of Agricultural Biotechnology. 2008, 16 (1): 24-31. Article.Chinese.

2.Xie S, Li X, Su L, Gu T, Zhao S. A Facile, Rapid and Effective Method for RNA Quality Determination. Journal of Agricultural Biotechnology. 2009, 17 (2):282-287. Article.Chinese.

3. Xie S, Huang T, Shen Y, Li X, Zhang X, Zhu M, Qin H, Zhao S. Identification and characterization of microRNAs from porcine skeletal muscle.Anim Genet. 2010,41(2):179-90. doi: 10.1111/j.1365-2052.2009.01991.x.

4. Xie S#, Li X#, Liu T, Cao J, Zhong Q, Zhao S. Discovery of porcine microRNAs in multiple tissues by a Solexa deep sequencing approach. PLoS One. 2011, 6(1):e16235. doi: 10.1371/journal.pone.0016235. (#co-first author)

5. Ma W, Xie S, Ni M, Huang X, Hu S, Liu Q, Liu A, Zhang J, Zhang Y. MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP). J Biol Chem. 2012, 287(13):10189-99. doi: 10.1074/jbc.M111.303636.

6．Sun N, Liu D, Chen H, Liu X, Meng F, Zhang X, Chen H, Xie S, Li X, Wu Z. Localization, expression change in PRRSV infection and association analysis of the porcine TAP1 gene. Int J Biol Sci. 2012, 8(1):49-58.doi:10.7150/ijbs.8.49.

7. Zhang J, Xie S, Ma W, Teng Y, Tian Y, Huang X, Zhang Y. A newly identified microRNA,mmu-miR-7578,functions as a negative regulator on inflammatory cytokines tumor necrosis factor-α and interleukin-6 via targeting Egr1 in vivo. J Biol Chem.2013, 288(6):4310-20.doi: 10.1074/jbc.M112.351197.

8. Xie S#, Xu J#, Ma W, Liu Q, Han J, Yao G, Huang X, Zhang Y. Lcn5 promoter directs the region-specific expression of cre recombinase in caput epididymidis of transgenic mice. Biol Reprod. 2013, 88(3):71.doi: 10.1095/biolreprod.112.104034. (#co-first author)

9. Ma W, Hu S, Yao G, Xie S, Ni M, Liu Q, Gao X, Zhang J, Huang X, Zhang Y. An androgen receptor-microrna-29a regulatory circuitry in mouse epididymis. J Biol Chem. 2013, 288(41):29369-81.doi: 10.1074/jbc.M113.454066.

10. Xie S, Shen B, Zhang C, Huang X, Zhang Y. sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One. 2014,9(6):e100448.doi: 10.1371/journal.pone.0100448.

11. Chu C, Zheng G, Hu S, Zhang J, Xie S, Ma W, Ni M, Tang C, Zhou L, Zhou Y, Liu M, Li Y, Zhang Y. Epididymal Region-Specific miRNA Expression and DNA Methylation and Their Roles in Controlling Gene Expression in Rats. PLoS One. 2015, 10(4):e0124450. doi: 10.1371/journal.pone.0124450.

12. Zhao C, Zhang Y, Li G, Chen J, Li J, Ren R, Ni P, Zhao S, Xie S*.Development of a graphical user interface for sgRNAcas9 and its application. Yi Chuan. 2015, 37(10):1061-72. doi: 10.16288/j.yczz.15-292. Article.Chinese.

13.Xie S, Zhang Y, Zhang L, Li G, Zhao C, Ni P, Zhao S.sgRNA design for the CRISPR/Cas9 system and evaluation of its off-target effects. Yi Chuan. 2015, 37(11):1125-36. doi: 10.16288/j.yczz.15-093. Review. Chinese.

14.Xie S*, Liu X, Zeng C, Ma Y, Wang H.The application of CRISPR/Cas9 technology in the innovative experimental teaching of molecular biology. Chemisty of life, 2016, 36(1): 117-125. doi: 10.13488/j.smhx.20160119. Article.Chinese.

15.Xu J, Yao G, Ru Y, Xie S*. Expression of tamoxifen-inducible CRE recombinase in Lcn5-CreERT2 transgenic mouse caput epididymis. Mol Reprod Dev. 2017, 84(3):257-264. doi:10.1002/mrd.22772.

16.Chen R, Du J, Ma L, Wang L, Xie S, Yang C, Lan X, Pan C, Dong W. Comparative microRNAome analysis of the testis and ovary of the Chinese giant salamander. Reproduction, 2017,154(3):169-179.doi: 10.1530/REP-17-0109.

17.Cheng Y, Wei Z, Xie S, Peng Y, Yan Y, Qin D, Liu S, Xu Y, Li G, Zhang L. Alleviation of Toxicity Caused by Overactivation of Pparα through Pparα-Inducible miR-181a2. Molecular Therapy-Nucleic Acids, 2017, 9:195-206.DOI:http://dx.doi.org/10.1016/j.omtn.2017.09.008.

18. Zhao C, Zheng X, Qu W, Li G, Li X, Miao Y, Han X, Liu X, Li Z, Ma Y, Shao Q, Li H, Sun F*, Xie S*, Zhao S*. CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif. Int J Biol Sci, 2017,13(11):1470-1478.d:10.7150/ijbs.21312. (*Co-Corresponding authors)

科研項目

1. 小鼠附睾piRNA時空表達特性及其生物學功能研究 2014.01

2. 基於CRISPR/CAS技術軟件開發及應用 2015.01

3. 抗病高產轉基因豬新品種培育 2016.01

4. 豬初始態（naive）多能幹細胞系建立及多能性調控機制解析 2016.06 ^[1] 

1.發明名稱：轉基因構建物及其在製備附睾頭部基因條件性敲除小鼠模型中的應用，授權號：ZL 201210507321.1。

2.發明名稱：一種檢測豬T細胞亞羣性狀的SNP分子標記，專利申請號：201510089345.3

3.發明名稱：DOCK2基因片段作為豬免疫性狀的分子標記及應用，專利申請號：201510447682

4.發明名稱：一種成熟體miRNA表達檢測引物設計新方法及其應用，專利申請號：2016107850720

5.軟件名稱：miRNA定量表達檢測引物和寡核苷酸探針設計軟件，版本號：V1.0，登記號：2015SR095326

6.軟件名稱：miRNA定量表達檢測引物和寡核苷酸探針設計軟件，版本號：V2.0，登記號：2015SR184550

7.軟件名稱：CRISPR/Cas9系統介導的基因組編輯技術sgRNA設計和脱靶效應評估軟件，版本號：V2.0，登記號：2015SR170234

8.軟件名稱：CRISPR/Cas9系統介導的RNA靶向編輯技術sgRNA設計和脱靶效應評估軟件，版本號：V1.0，登記號：2015SR285305

9.軟件名稱：基於CRISPR-Cas9/Cpf1/C2c1系統介導的基因組靶向編輯技術sgRNA設計和脱靶效應評估軟件,版本號：V1.0，登記號：2016SR040793

10.軟件名稱：sgRNA表達載體測序序列同源比對工具，版本號：V1.0，登記號：2016SR232809。 ^[1]