袁凌

1989年在德克薩斯大學奧斯丁分校獲得生物化學與分子生物學博士學位 ^[4] 

目前任職肯塔基大學植物與土壤科學系終身教授，並擔任肯塔基大學煙草研究與發展中心主任和首席科學家，同時也是山西省“131”領軍人才工程特聘專家。 ^[4]  中國科學院“植物資源保護與可持續利用”創新團隊計劃項目的美方專家。 ^[5] 

袁凌教授的研究領域涉及蛋白質工程、定向進化、生物能源和代謝途徑工程（Proteinengineering, directed evolution, bioenergy and metabolic pathway engineering）。袁凌教授現主持美國農業部（USDA）、美國能源部（DOE）、肯塔基州能源政策部和肯塔基州煙草研究與發展中心（KTRDC）的多個科研項目。 ^[4] 

近年來在New Phytologist, Plant Physiology, Nature Biotechnology， Microbiologyand Molecular Biology Reviews，Proceeding of National Academy of Science U.S.A.，Current Opinion in Biotechnology，Journal of Biological Chemistry等國際頂尖學術雜誌上發表論文近40篇。 ^[4] 

Protein engineering, directed evolution, metabolic pathway engineering

1989, Ph.D., University of Texas at Austin, Biochemistry and molecular biology

1983, B.S. Jinan University, Guangzhou, China, Biology

2003 - present, Associate Professor, Department of Plant and Soil Sciences, University of Kentucky

1998 - 2003, Program Manager, Maxygen, Inc. Redwood City, California

1993 - 1998, Principal Scientist, Calgene/Monsanto, Davis, California

1990 - 1993, Assistant Specialist, University of California at San Francisco

1988 - 1990, Postdoctoral fellow, Pharmaceutical Chemistry Department, University of California at San Francisco

This person page last updated on 2011-06-14

Pattanaik, S., Werkman, JR., Kong, Q. and Ling Yuan, 2009. Site-directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis. Metabolic Engineering of Plant Secondary Pathways. in Methods in Molecular Biology. (Book chapter; in press)

Bai, Y.H., Pattanaik, S., Patra, B., Werkman, J.R., Xie, C.H. and Yuan, L. 2011. Flavonoid-related basic helix-loop-helix regulator, NtAn1a and NtAn1b, of tobacco have originated from two ancestors and are functionally active. Planta. (in press)

Jordan, D.B., Wagschal, K., Fan, ZM, Yuan, L., Braker, J.D. and Heng, C. 2011. Engineering lower inhibitor affinities in beta-D-xylosidase of Selenomonas ruminantium by site-directed mutagenesis of Trp145. Journal of Industrial Microbiology and Biotechnology. (in press)

Fan, ZM and Ling Yuan. 2010. Production of Multifunctional Chimeric Enzymes in Plants: a promising Approach for Degrading Plant Cell Wall from Within. Plant Biotechnology Journal. 8:308-315

Fan, ZM., Yuan, L., Jordan, DB., Wagschal, K., Heng, C., Braker, JD. 2010. Engineering Lower Inhibitor Affinities in Beta-D-xylosidase. Applied Microbiology and Biotechnology. 86(4):1099-1113

Pattanaik, S., Kong, Q., Zaitlin, D., Werkman, JR., Xie, CH., Patra, B., and Ling Yuan, 2010. Isolation and functional characterization of a floral tissue-specific R2R3 MYB regulator from tobacco. Planta. 231(5):1061-1076

Fan, Z.M., K. Wagschal, C.C. Lee, Q. Kong, K.A. Shen, I.B. Maiti, and L. Yuan. 2009. The construction and characterization of two xylan-degrading chimeric enzymes. Biotechnology and Bioengineering 102:684- 692.

Fan, Z.M., J.H. Werkman, and L. Yuan. 2009. Engineering a multifunctional hemicellulase. Biotechnology Letters 31:751-757.

Fan, Z.M., L. Yuan, D.B. Jordan, K. Wagschal, C. Heng, and J.D. Braker. 2009. Engineering lower inhibitor affinities in B-D-xylosidase. Applied Microbiology and Biotechnology. Published online: doi: 10.1007/s00253-009-2335-7.

Fan, ZM, Ling Yuan, Chatterjee, R. 2009. Increased Hydrogen Production by Genetic Engineering of Escherichia coli. PLoS ONE 4(2): e4432

Fan, ZM., Wagschal, K., Chen, W., Montross, MD., Lee, CC, and Ling Yuan, 2009. Multimeric Hemicellulases Facilitate Biomass Conversion. Applied and Environmental Microbiology. 75: 1754-1757

Fan, ZM., Wagschal, K., Lee, C.C., Kong, Q., Shen, K.A., Maiti, I.B., and Ling Yuan, 2009. The Construction and Characterization of Two Xylan-degrading Chimeric Enzymes. Biotechnology and Bioengineering. 102(3): 684-692

Fan, ZM., Werkman, J.R. and Ling Yuan, 2009. Engineering of a Multifunctional Hemicellulase. Biotechnology Letters. 31: 751-757

Pattanaik, S., C.H. Xie, and L. Yuan. 2008. The interaction domains of the plant Myc-like bHLH transcription factors can regulate the transactivation strength. Planta 227:707-715.

Authors in PSS Dept.: Ling Yuan Sitakanta Pattanaik

Pattanaik, S., C.H. Xie and L. Yuan 2008. The Interaction Domains of the Plant Myc-like bHLH Transcription Factors Can Regulate the Transcription Strength. Planta. 227(3): 707-715

Pattanaik, S., C.H. Xie, and L. Yuan. 2008. Creation of master transcription factors to regulate plant metabolic pathways. Acta Horticulturae 756:109-118.

Authors in PSS Dept.: Ling Yuan Sitakanta Pattanaik

Zeng, Q., F. Qiu, and L. Yuan. 2008. Production of artemisinin by genetically modified microbes. Biotechnology Letters 30:581-592.

Zeng, QP, F. Qiu and L. Yuan 2008. Production of Artemisinin by Genetically-modified Microbes. Biotechnol Lett 30: 581-592

Pattanaik, S., C.H. Xie and L. Yuan 2007. Creation of Master Transcription Factors to Regulate Plant Metabolic Pathways. Acta Hort. 756: 109-118

Suttipanta, N., Pattanaik, S., Gunjun, S., Xie, CH, Littleton, J. and Yuan, L. 2007. Promoter analysis of the Catharanthus roseus geraniol 10-hydroxylase gene involved in terpenoid indole alkaloid biosynthesis. Biochim Biophys Acta, 1769:139-148

Suttipanta, N., S. Pattanaik, S. Gunjan, J. Littleton, and L. Yuan. 2007. Promoter analysis of the Catharanthus roseus geranial 10-hydroxylase gene involved in terpenoid indole alkaloid biosynthesis. Biochemica et Biophysica Acta 1769:139-148.

Authors in PSS Dept.: Ling Yuan Sitakanta Pattanaik

Chatterjee, R., and L. Yuan. 2006. Directed evolution of metabolic pathways. Trends in Biotechnology. 24(1): 28-38

Pattanaik, S., C.H. Xie, Q. Kong, K.A. Shen, and L. Yuan 2006. Directed evolution of plant helix-loop-helix transcription factors for the improvement of transactivation properties. Biochim Biophys Acta, 1759: 308-318

Yuan, L., I. Kurek, J. English and R. Keenan. 2005. Laboratory-directed protein evolution. Microbiology and Molecular Biology Reviews. 69(3): 373-392

Zheng W, Wise ML, Wyrick A, Metz JG, Yuan L, Gerwick WH. 2002. Polyenoic fatty acid isomerase from the marine alga Ptilota filicina: protein characterization and functional expression of the cloned cDNA. Arch Biochem Biophys. 401(1):11-20.

Facciotti, M., Burtain, P., and Ling Yuan 1999. Improved Stearate Phenotype in Transgenic Canola Expressing a Modified Acyl-acyl Carrier Protein Thioesterase. Nature Biotechnology. 17 (6): 593-597.

Huang YS, Chaudhary S, Thurmond JM, Bobik EG Jr, Yuan L, Chan GM, Kirchner SJ, Mukerji P, Knutzon DS. 1999. Cloning of delta12- and delta6-desaturases from Mortierella alpina and recombinant production of gamma-linolenic acid in Saccharomyces cerevisiae. Lipids 34(7):649-59.

Facciotti, M. T. andLing Yuan, 1998. Molecular Dissection of the Plant ACP-thioesterases. Fett/Lipid 100 (4-5): 167-172.

Knauf, V.C and Ling Yuan, 1997. Modification of Plant Components. Current Opinion in Biotechnology. 8: 227-233.

Ling Yuan, B. A. Nelson, and G. Caryl. 1996. The Catalytic Cysteine and Histidine in the Plant Acyl-ACP Thioesterases. Journal of Biological Chemistry 271: 3417-3419.

Ling Yuan, T. A. Voelker and D. J. Hawkins 1995. Modification of the Substrate Specificity of an Acyl-ACP Thioesterase by Protein Engineering. Proceeding of National Academy of Science U.S.A. 92: 10639-10643.