毛勝勇

1. 反芻動物瘤胃酸中毒的發生機制及營養調控研究

系統開展了反芻動物瘤胃酸中毒的發生機制及營養調控研究，闡明瞭瘤胃酸中毒發病的微生物學機制，揭示了瘤胃乳酸積累的微生物作用機制，為瘤胃酸中毒的定向調控提供了重要理論依據。建立了基於DGGE和16S rRNA序列分析瘤胃微生物區繫結構的技術手段，研究了日糧結構對瘤胃發酵特性及細菌區系的影響，進行了乳酸利用菌的分離及其對瘤胃發酵的影響的研究。

2.瘤胃產甲烷的微生物分子特徵及調控措施的研究

闡明瞭瘤胃產甲烷的機制，提出了相應的調控措施，為提高飼料利用率、減少甲烷對環境污染奠定了理論基礎。建立了瘤胃甲烷菌、厭氧真菌多樣性和定量分析技術， 建立了晉南牛瘤胃產甲烷菌16S rRNA基因序列庫，並研究了甲烷菌菌羣分佈及多樣性。

獲得1項發明專利，申請國家發明專利2項。發表論文30篇，其中SCI 收錄文章7篇。

1. Cai-Xia Pei, Sheng-Yong Mao, Yan-Fen Cheng and Wei-Yun Zhu, Diversity, abundance and novel 16S rRNA gene sequences of methanogens in rumen liquid, solid and epithelium fractions of Jinnan cattle. Animal, 2010, 4:1, 20–29

2. Yun Zhang Sun, Sheng-Yong Mao, Wei-Yun Zhu. Rumen chemical and bacterial changes during stepwise adaptation to a high concentrate diet in goats. Animal ,2010, 4: 210-217

3. Y.F. Cheng, S.Y. Mao, J.X. Liu and W.Y. Zhu. Molecular diversity analysis of rumen methanogenic Archaea from goat in eastern China by DGGE methods using different primer pairs. Letters in Applied Microbiology, 2009, 48(5):585-592.

4. Mao SY, Zhang G, Zhu WY, Effect of disodium fumarate on ruminal metabolism and rumen bacterial communities in goat as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA. Animal Feed Science and Technology, 2008, 140: 293–306.

5. Sun YZ, Mao SY, Yao W, Zhu WY. DGGE and 16S rDNA analysis reveals a highly diverse and rapidly colonising bacterial community on different substrates in the rumen of goats. Animal, 2008, 2 (3): 391–398

6. Mao SY, Zhu WY, Wang QJ, Yao W.2007, Effect of daidzein on in vitro fermentation of micro-organisms from the goat rumen. Animal Feed Science and Technology, 136：154-163.

7. Mao SY, Zhang G, Zhu WY, 2007, Effect of disodium fumarate on in vitro rumen fermentation of different substrates and rumen bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA. Asia-Australian Journal of Animal Sciences, 20 (4): 543-549.

8. Liu W, Mao SY, Zhu WY, 2007. Impact of tiny miRNAs on cancers. World Journal of Gastroenterology, 13(4):497-502.

9. Zhu WY, Mao SY, Liu JX, Cheng YF, Iqbal MF, Wang JK, Diversity of methanogens and their interactions with other microorganisms in methanogenesis in the rumen. Plenary Paper, Proceedings of the 7th International Symposium on the Nutrition of Herbivores, pp 125-139, Beijing, September 17 – 22, 2007

10. Zhu W-Y, Mao SY, Yao W. (2003) Influence of anaerobic fungi on the fermentation of rice straw by rumen microbial populations. Tropical and Subtropical Agroecosystems 3:191-196.