楊淑華

1987年9月－1991年6月，南開大學，理學學士學位

1991年9月－1994年6月，南開大學，理學碩士學位

1998年7月－2002年1月，新加坡國立大學生物科學系，博士學位 ^[1] 

1994年7月－1998年6月，南開大學元素有機化學研究所，研究實習員、助理研究員

2002年2月－2004年12月，美國康奈爾大學植物科學系，博士後

2005年1月－今，中國農業大學生物學院，教授 ^[1] 

九三學社第十五屆中央委員會農林專門委員會副主任。 ^[6] 

植物響應低温脅迫的分子機制

作為外界主要環境因子，温度影響植物的生長髮育、植被的分佈以及農作物的產量。我們實驗室主要是通過分子生物學、遺傳學和生物化學等手段，研究植物應答低温脅迫的信號轉導途徑、低温信號與植物激素信號之間的交互作用，以及低温信號與生物脅迫信號之間的交互作用等。 ^[2] 

在植物抗凍性研究方面，我們建立了穩定的凍相關突變體的篩選體系。通過該體系，我們發現蛋白激酶CRPK1（Cold-responsive protein kinase 1）參與負向調控低温信號途徑關鍵轉錄因子CBF依賴的信號轉導。低温激活定位於細胞膜上的CRPK1，後者磷酸化細胞質中的14-3-3蛋白，誘導其進入細胞核中，與CBF1/3蛋白互作，介導CBF1/3蛋白的泛素化降解，從而負調控植物對低温的應答。該研究不僅揭示了低温信號從細胞膜傳遞到細胞核中的負調控機制，也闡明瞭CBF蛋白翻譯後修飾的調控機制（Liu et al., 2017,Mol Cell）。而蛋白激酶OST1在植物低温應答中發揮重要的正向調控作用。OST1激酶活性被低温迅速誘導，激活狀態的OST1將ICE1磷酸化，抑制ICE1的泛素化降解，從而正調控植物的抗凍性（Ding et al., 2015, Dev Cell）。

在低温信號和植物激素信號的交叉互作方面，我們發現乙烯信號通路關鍵組分均參與低温應答。轉錄因子EIN3蛋白直接負調控低温調節基因CBF和細胞分裂素信號負調控因子A型ARR基因的表達，從而負調控擬南芥的抗凍性。該研究明確了乙烯在植物抗低温中的作用，同時也揭示乙烯和細胞分裂素信號之間交互作用的分子機制（Shi et al., 2012, Plant Cell）。另一個重要植物激素油菜素內酯信號通路關鍵的蛋白激酶BIN2和轉錄因子BZR1/BES1通過依賴和不依賴於CBF的方式調控下游一系列基因的表達，從而正向調節植物的抗凍能力（Li et al., 2017, Mol Plant）。利用CRISPR技術，構建了cbfs三缺失突變體，明確了CBFs在低温馴化中的關鍵作用及CBFs的靶基因，併為低温信號研究提供了理想的遺傳材料 (Jia et al., 2016, New Phytol)。

我們的研究發現擬南芥防衞反應中起重要作用的抗病相關基因CHS1、RPP4/CHS2、CHS3和LSD1/CHS4參與不同温度下植物生長的穩態平衡調控 (Wang et al., 2013, Plant J; Wang et al., 2011, Plant J; Huang et al., 2010, Plant Physiol; Yang et al., 2010, Plant J; Huang et al., 2010,New Phytol)。研究揭示TIR-NB類蛋白CHS1通過與R蛋白SOC3互作，蛋白磷酸酶IBR5和伴侶蛋白HSP90分別與R蛋白CHS3和CHS2互作，調控R蛋白的活性，從而調控R介導的温度依賴的細胞死亡和防衞反應 (Zhang et al., 2016, New Phytol; Liu et al., 2015, PLoS Genet; Bao et al., 2014, New Phytol)。

研究發現擬南芥磷脂結合蛋白BON1在調控温度依賴的生長髮育中是一個關鍵調控因子，BON1與兩個類受體蛋白激酶BIR1和BAK1互作，可能通過BAK1對BIR1和BON1的磷酸化，作用於同一條信號通路，協同調控温度依賴的植物生長髮育和防衞反應 (Wang et al., 2011, Plant J; Yang et al., 2007, Plant Physiol; Yang et al., 2006, Plant J; Yang and Hua, 2004, Plant Cell)。 ^[3] 

本科生 《植物生理學》

研究生 《高級植物生理學與分子生物學》、《植物學Seminar》 ^[1] 

Ding Y#, Shi Y#, Yang S* (2019) Advances and challenges in uncovering cold tolerance regulatory mechanisms in plants. New Phytol (invited Tansley Review) Doi: 10.1111/nph.15696.

Wang X, Guo C, Peng J, Li C, Wan F, Zhang S, Zhou Y, Yan Y, Qi L, Sun K, Yang S, Gong Z, Li J* (2019) ABRE-BINDING FACTORS play a role in the feedback regulation of ABA signaling by mediating rapid ABA induction of ABA co-receptor genes. New Phytol 221: 341-355.

Feng Y, Liu J, Zhai L, Gan Z, Zhang G, Yang S, Wang Y, Wu T, Zhang X, Xu X, Han Z* (2019) Natural variation in cytokinin maintenance improves salt tolerance in apple rootstocks. Plant Cell Environ 42: 424-436.

Ding Y, Lv J, Shi Y, Gao J, Hua J, Song C, Gong Z, Yang S* (2019) EGR2 phosphatase regulates OST1 kinase activity and freezing tolerance in Arabidopsis. EMBO J 38: e99819. (Selected for F1000 Prime)

Shi Y#, Ding Y#, Yang S* (2018) Molecular regulation of CBF signaling in cold acclimation. Trends Plant Sci (invited review) 23: 623-637.

Ding Y, Jia Y, Shi Y, Song C, Gong Z, Yang S* (2018) OST1-mediated BTF3L phosphorylation positively regulates CBFs during plant cold responses. EMBO J 37: e98228.

Liu J#, Shi Y#, Yang S* (2018) Insights into the regulation of C-repeat binding factors in plant cold signaling. J Integr Plant Biol (invited review) 60: 780-795.

Huang X, Hou L, Meng J, You H, Li Z, Gong Z, Yang S, Shi Y*(2018) The antagonistic action of abscisic acid and cytokinin signaling mediates drought stress response in Arabidopsis. Mol Plant 11: 970-982.

Wang K#, He J#, Zhao Y, Wu T, Zhou X, Ding Y, Kong L, Wang X, Wang Y, Li J, Song CP, Wang B, Yang S, Zhu JK, Gong Z* (2018) EAR1 negatively regulates ABA signaling by enhancing 2C protein phosphatase activity. Plant Cell 30: 815-834.

Wei D, Liu M, Chen H, Zheng Y, Liu Y, Wang X, Yang S, Zhou M*, Lin J* (2018) INDUCER OF CBF EXPRESSION 1 is a male fertility regulator impacting anther dehydration in Arabidopsis. PLoS Genet 14: e1007695.

Li H, Ding Y, Shi Y, Zhang X, Zhang S, Gong Z, Yang S* (2017) MPK3- and MPK6-mediated ICE1 phosphorylation negatively regulates ICE1 stability and freezing tolerance in Arabidopsis. Dev Cell 43: 630-642. (Highlighted with a Preview article in Dev Cell and a Spotlight article in Trends Plant Sci; Selected for F1000 Prime; 植物學報熱點評)

Jiang B#, Shi Y#, Zhang X, Xin X, Qi L, Guo H, Li J*, Yang S* (2017) PIF3 is a negative regulator of the CBF pathway and freezing tolerance in Arabidopsis. Proc Natl Acad Sci USA 114: E6695-E6702. (Highlighted with a News & Views in Nat Plant and a Spotlight article in Trends Plant Sci)

Liu Z, Jia Y, Ding Y, Shi Y, Zhang X, Li Z, Guo Y, Gong Z, Yang S* (2017) Plasma membrane CRPK1-mediated phosphorylation of 14-3-3 proteins induces their nuclear import to fine-tune CBF signaling during cold response. Mol Cell 66: 117-128. (Highlighted with a Preview article in Mol Cell; Selected for F1000 Prime)

Li H#, Ye K#, Shi Y, Zhang X, Yang S* (2017) BZR1 positively regulates freezing tolerance via CBF-dependent and CBF-independent pathways in Arabidopsis. Mol Plant 10: 545-559. (Highlighted with a Spotlight article in Mol Plant)

Zhang Z#, Li J#, Pan Y#, Li J#, Zhou L#, Shi H#, Zeng Y#, Guo H, Yang S, Zheng W, Yu J, Sun X, Li G, Ding Y, Ma L, Shen S, Dai L, Zhang H, Yang S, Guo Y, Li Z* (2017) Natural variation in CTB4a enhances rice adaptation to cold habitats. Nat Commun 8:14788.

Zhang Y#, Wang Y#, Liu J, Ding Y, Wang S, Zhang X, Liu Y, Yang S* (2017) Temperature-dependent autoimmunity mediated by chs1 requires its neighboring TNL gene SOC3. New Phytol 213:1330-1345.

Tong M, Kotur T, Liang W, Vogelmann K, Kleine T, Leister D, Brieske C, Yang S, Lüdke D, Wiermer M, Zhang Y, Li X*, Hoth S*. (2017) E3 ligase SAUL1 serves as a positive regulator of PAMP-triggered immunity and its homeostasis is monitored by immune receptor SOC3. New Phytol 215:1516-1532.

Huang X, Zhang X, Gong Z, Yang S, Shi Y* (2017) ABI4 represses the expression of type-A ARRs to inhibit seed germination in Arabidopsis. Plant J 89: 354-365.

Jia Y, Ding Y, Shi Y, Zhang X, Gong Z, Yang S* (2016) The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis. New Phytol 212: 345-353. (Highlighted with a Commentary article in New Phytol)

Yu F, Lou L, Tian M, Li Q, Ding Y, Wu Y, Belda-Palazon B, Rodrigue P L, Yang S, Xie Q* (2016) ESCRT-I component VPS23A affects ABA signaling by recognizing ABA receptors for endosomal degradation. Mol Plant 9: 1570-1582.

Ding Y, Li H, Zhang X, Xie Q, Gong Z, Yang S* (2015) OST1 kinase modulates freezing tolerance by enhancing ICE1 stability in Arabidopsis. Dev Cell 32: 278-289. (Highlighted with a Preview article in Dev Cell; Highlighted with an article in SCIENCE CHINA Life Sciences; 植物學報熱點評)

Liu J#, Yang H#, Bao F, Ao K, Zhang X, Zhang Y, Yang S* (2015) IBR5 modulates temperature-dependent, R protein CHS3-mediated defense responses in Arabidopsis. PLoS Genet 11, e1005584.

Kong L, Cheng J, Zhu Y, Ding Y, Meng J, Chen Z, Xie Q, Guo Y, Li J, Yang S, Gong Z* (2015) Degradation of the ABA co-receptor ABI1 by PUB12/13 U-box E3 ligases. Nat Commun 6: 8630. doi: 10.1038/ncomms9630.

Ji H, Wang Y, Cloix C, Li K, Jenkin G, Wang S, Shang Z, Shi Y, Yang S, Li X* (2015) The Arabidopsis RCC1 family protein TCF1 regulates freezing tolerance and cold acclimation through modulating lignin biosynthesis. PLoS Genet 11, e1005471.

Shi Y#, Ding Y#, Yang S* (2015) Cold signal transduction and its interplay with phytohormones during cold acclimation. Plant Cell Physiol (invited review) 56: 7-15.

Bao F, Huang X, Zhu C, Zhang X, Li X, Yang S* (2014) Arabidopsis HSP90 protein modulates RPP4-mediated temperature-dependent cell death and defense responses. New Phytol 202: 1320-1334.

Song S, Huang H, Gao H, Wang J, Wu D, Liu X, Yang S, Zhai Q, Li C, Qi T, Xie D* (2014) Interaction between MYC2 and ETHYLENE INSENSITIVE3 modulates antagonism between jasmonate and ethylene signaling in Arabidopsis. Plant Cell 26: 263-279.

Wang Y, Zhang Y, Wang Z, Zhang X, Yang S* (2013) A missense mutation in CHS1, a TIR-NB protein, induces chilling sensitivity in Arabidopsis. Plant J 75: 553-565.

Shi Y, Wang Z, Meng P, Tian S, Zhang X, Yang S* (2013) The glutamate carboxypeptidase AMP1 mediates ABA and abiotic stress responses in Arabidopsis. New Phytol 199: 135-150.

Guo L, Yang H, Zhang X, Yang S* (2013) Lipid transfer protein 3 as a target of MYB96 mediates freezing and drought stress in Arabidopsis. J Exp Bot 64: 1755-1767.

Li J#, Mu J#, Bai J#, Fu F, Zou T, An F, Zhang J, Jing H, Wang Q, Yang S, Zuo J* (2013) PARAQUAT RESISTANT 1, a Golgi-localized putative transporter protein, is involved in intracellular transport of paraquat. Plant Physiol 162: 470-483.

Shi Y#, Tian S#, Hou L, Huang X, Zhang X, Guo H, Yang S* (2012) Ethylene signaling negatively regulates freezing tolerance by repressing expression of CBF and type-A ARR genes in Arabidopsis. Plant Cell 24: 2578-2595.

Wang Z#, Meng P#, Zhang X, Ren D, Yang S* (2011) BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature-dependent plant growth and cell death in Arabidopsis. Plant J 67: 1081-1093.

Huang X, Li J, Bao F, Zhang X, Yang S* (2010) A gain-of-function mutation in the Arabidopsis disease resistance gene RPP4 confers sensitivity to low temperature. Plant Physiol 154: 796-809.

Yang H, Shi Y, Liu J, Guo L, Zhang X, Yang S* (2010) A mutant CHS3 protein with TIR-NB-LRR-LIM domains modulates growth, cell death and freezing tolerance in a temperature-dependent manner in Arabidopsis. Plant J 63: 283-296.

Huang X, Li Y, Zhang X, Zuo J, Yang S* (2010) The Arabidopsis LSD1 gene plays an important role in the regulation of low temperature-dependent cell death. New Phytol 187: 301-312 (Cover story).

Liu D, Gong Q, Ma Y, Li P, Li J, Yang S, Yuan L, Yu Y, Pan D, Xu F, Wang NN* (2010) cpSecA, a thylakoid protein translocase subunit, is essential for photosynthetic development in Arabidopsis. J Exp Bot 61: 1655-1669.

Huang X, Zhang X, Yang S* (2009) A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis. Cell Res 19: 1205-1216.

Gou M, Su N, Zheng J, Huai J, Wu G, Zhao J, He J, Tang D, Yang S, Wang G* (2009) An F-box gene, CPR30, functions as a negative regulator of the defense response in Arabidopsis. Plant J 60: 757-770.

Li Y#, Yang S#, Yang H, Hua J* (2007) The TIR-NB-LRR gene SNC1 is regulated at the transcript level by multiple factors. Mol Plant Microbe Interact 20: 1449-1456.

Yang H, Yang S, Li Y, Hua J* (2007) The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death. Plant Physiol 145: 135-146.

Chai MF, Wei PC, Chen QJ, An R, Chen YM, Chen J, Yang S*, Wang XC* (2006) NADK3, a novel cytoplasmic source of NADPH, is required under conditions of oxidative stress and modulates abscisic acid responses in Arabidopsis. Plant J 47: 665-674.

Yang S, Yang H, Grisafi P, Sanchatjate S, Fink GR, Sun Q, Hua J* (2006) The BON/CPN gene family represses cell death and promotes cell growth in Arabidopsis. Plant J 45: 166-179.

Yang S, and Hua J* (2004) A haplotype-specific resistance gene regulated by BONZAI1 mediates temperature-dependent growth control in Arabidopsis. Plant Cell 16: 1060-1071.

Yang S*, Yu H, Xu Y, Goh CJ* (2003) Investigation of cytokinin-deficient phenotypes in Arabidopsis by ectopic expression of orchid DSCKX1. FEBS Lett 555: 291-296.

Yang S, Yu H, Goh CJ* (2003) Functional characterisation of a cytokinin oxidase gene DSCKX1 in Dendrobium orchid. Plant Mol Biol 51: 237-248.

Yang S, Yu H, Goh CJ* (2002) Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter. J Exp Bot 53: 1899-1907.

Yu H, Yang S, Goh CJ* (2002) Spatial and temporal expression of the orchid floral homeotic gene DOMADS1 is mediated by its upstream regulatory elements. Plant Mol Biol 49: 225-237.

Yu H, Yang S, Goh CJ* (2001) Agrobacterium-mediated transformation of a Dendrobium orchid with the class 1 knox gene DOH1. Plant Cell Rep 20: 301-305.

Yu H, Yang S, Goh CJ* (2000) DOH1, a class 1 knox Gene, is required for maintenance of the basic plant architecture and floral transition in orchid. Plant Cell 12: 2143-2160.

劉靜妍，施怡婷，楊淑華* (2017) CBF：平衡植物低温應答與生長髮育的關鍵. 植物學報. 52: 689-698.

丁楊林，施怡婷，楊淑華* (2015) 植物響應低温脅迫的分子機制研究. 生命科學. 27: 398-405.

鄧 巖，王興春，楊淑華*，左建儒* (2006) 細胞分裂素：代謝、信號轉導、交叉反應與農藝性狀改良. 植物學通報. 23: 478-498. ^[1] 

Shi Y, Yang S* (2014) ABA regulation of the cold stress response in plants. In Zhang D (ed) Abscisic Acid: Metabolism, Transport and Signaling. Springer, pp337-363.

Yu H, Dhavale T, Yang S (2006) Molecular mechanisms of hormone functions in flowering In Teixeira da Silva JA (ed) Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Ed 1 Vol 1 Global Science Books, London, UK, pp 25-32.

任勃，王興春，馮健，楊淑華，左建儒 (2012) 細胞分裂素. 植物激素作用的分子機理 (許智宏，薛紅衞主編) 上海科學技術出版社.

武維華，張蜀秋，袁明，張軍，韓玉珍，李穎章，葉德，楊淑華，王學臣 (2008) 植物生理學 (第二版) 科學出版社. ^[3] 

北京市第十六屆人民代表大會代表。 ^[5] 

中國植物學會副秘書長。 ^[7] 

2005年入選教育部“新世紀優秀人才支持計劃”

2012年獲得國家傑出青年科學基金”資助

2014年被聘為教育部“長江學者”特聘教授

2014年國務院政府津貼

2018年北京市三八紅旗獎章稱號 ^[1] 

2023年5月26日，被授予第三屆全國創新爭先獎狀。 ^[4]