於曉強

2000年開始從事雙光子方面的工作，在雙光子聚合方面已經取得了較大的成績。目前的工作集中在雙光子生物熒光探針的設計、製備和生物應用研究。此領域是一項多學科密集交叉的國際前沿領域，正在力爭在該領域作出創造性的工作。經過十年的建設，實驗室擁有先進有機材料製備、熒光性能表徵、雙光子性能測試、活性生物細胞與組織培育、生物熒光顯微成像等一流設備。特別是雙光子熒光測試系統、雙光子生物熒光顯微系統是我們根據生物熒光探針的實驗要求自行建設的，並得到了國際國內同行的認可，我們的實驗數據已經為浙江大學、中國科學院化學研究所、吉林大學、中國海洋大學、重慶大學等單位提供數據，並協助其發表論文。

(1) 雙光子光聚合反應的研究 國家自然科學基金委員會

(2) 聚合物型三維光子晶體的激光微加工和微型光學器件研究 國家自然科學基金委員會/香港資助局聯合資助

(3) 生物學光學窗口的水溶性雙光子熒光材料研究及相關核酸探針的探索 國家自然科學基金委員會

(4) 光波段具有負折射特性的特殊週期介質結構的研究 科技部973基礎研究課題

(5)雙光子DNA探針的研製 濟南試豪生物公司自主專利資助課題

Xuechen Li,Minggang Tian, Ge Zhang, Ruoyao Zhang, Ruiqing, Feng, Lifang Guo，Xiaoqiang Yu*, Ning Zhao*, Xiuquan He*. Aspatial-dependent fluorescent probe for detecting different situations ofmitochondrial membrane potential conveniently and efficiently. Anal. Chem. 2017.2.14,DOI: 10.1021/acs.analchem.6b03842.

1. Minggang Tian,Yong Liu, Yuming Sun, Ruoyao Zhang, Ruiqing Feng, Ge Zhang, Lifang Guo, XuechenLi,Xiaoqiang Yu*, Jing Zhi Sun*,Xiuquan He*. A single fluorescent probe enables clearly discriminating andsimultaneously imaging liquid-ordered and liquid-disordered microdomains inplasma membrane of living cells. Biomaterilas 2017, 12, 46~56.

2. Minggang Tian,Yuming Sun, Lifang Guo, Ruoyao Zhang, Ge Zhang, Ruiqing Feng, Xuechen Li,Huamiao Zhang, Ge Li，Xiaoqiang Yu*,XiuQuan He*. A small-structure two-photon platform equipped with weakelectrondonor: Development of permeable two-photon probes for lysosomesandmitochondria in living cells. Sensors and Actuators B 2017, 243, 955~962.

3. Lifang Guo,Ruoyao Zhang, Yuming Sun, Minggang Tian, Ge Zhang, Ruiqing Feng, Xuechen Li,Huamiao Zhang, Li He,Xiaoqiang Yu*,Xiuquan He*. Styrylpyridine salts-based red emissive two-photon turn-on probefor imaging the plasma membrane in living cells and tissues. Analyst 2016,141(11), 3228~3232.

4. Ge Zhang,Yuming Sun, Xiuquan He, Weijia Zhang, Minggang Tian, Ruiqing Feng, RuoyaoZhang, Xuechen Li, Lifang Guo,XiaoqiangYu*, Shangli Zhang*. Red-Emitting Mitochondrial Probe with UltrahighSignal-to-Noise Ratio Enables High-Fidelity Fluorescent Images in Two-PhotonMicroscopy. Analytical Chemistry 2015, 87(24), 12088~12095.

5. Ruiqing Feng,Yuming Sun, Minggang Tian, Ge Zhang, Ruoyao Zhang, Lifang Guo, Xuechen Li,Xiaoqiang Yu*, Ning Zhao*. Amembrane-permeable dye for living cells with large two-photon excitedfluorescence action cross-sections for bioimaging. Journal of MaterialsChemistry B 2015, 3(44), 8644~8649.

6. Guofen Song,Yuming Sun, Yong Liu, Xiankun Wang, Meiling Chen, Fang Miao, Weijia Zhang,Xiaoqiang Yu*, Jianling Jin*. Lowmolecular weight fluorescent probes with good photostability for imagingRNA-rich nucleolus and RNA in cytoplasm in living cells. Biomaterials 2014,35(7), 2103~2112.

7. Fang Miao,Weijia Zhang, Yuming Sun, Ruoyao Zhang, Yong Liu, Fuqiang Guo, Guofen Song,Minggang Tian,Xiaoqiang Yu*. Novelfluorescent probes for highly selective two-photon imaging of mitochondria inliving cells. Biosens. Bioelectron. 2014, 55(15), 423~429.

8. Minggang Tian,Fuqiang Guo, Yuming Sun, Weijia Zhang, Fang Miao, Yong Liu, Guofen Song, Cheuk-LamHo,Xiaoqiang Yu,* Jing Zhi Sun* andWai-Yeung Wong*. A fluorescent probe for intracellular cysteine overcoming theinterference by glutathione. Org. Biomol. Chem., 2014, 12(32), 6128~6133.

9. Yong Liu,Weijia Zhang, Yuming Sun, Guofen Song, Fang Miao, Fuqiang Guo, Minggang Tian,Xiaoqiang Yu*, Jing Zhi Sun*.Two-photon fluorescence imaging of RNA in nucleoli and cytoplasm in livingcells based on low molecular weight probes. Dyes and Pigments, 2014, 103(6), 191~201.

10. Junjie Wang,Yuming Sun, Weijia Zhang, Yong Liu,XiaoqiangYu*, Ning Zhao* A two-photon ratiometric fluorescent probe enables spatialcoordinates determination of intracellular pH. Talanta, 2014, 129, 241~248.

11. Fang Miao,Guofen Song, Yuming Sun, Yong Liu, Fuqiang Guo, Weijia Zhang, Minggang Tian,Xiaoqiang Yu*. Fluorescent imaging ofacidic compartments in living cells with a high selective novel one-photonratiometric and two-photon acidic pH probe. Biosens. Bioelectron. 2013, 50(4) 42~49.

12. Fuqiang Guo,Minggang Tian, Fang Miao, Weijia Zhang, Guofen Song, Yong Liu,Xiaoqiang Yu*, Jing Zhi Sun*, Wai-YeungWong*. Lighting up cysteine and homocysteine in sequence based on the kineticdifference of the cyclization/addition reaction. Org. Biomol. Chem., 2013,11(44), 7721~7728.

13. Zhiguang Yang,Ning Zhao, Yuming Sun, Fang Miao,Yong Liu, Xin Liu, Yuanhong Zhang, Wentao Ai,Guofen Song, Xiaoyuan Shen,Xiaoqiang Yu*,Jingzhi Sun* and Wai-Yeung Wong*. Highly selective red- and green-emittingtwo-photon fluorescent probes for cysteine detection and their bio-imaging inliving cells. Chem. Commun. 2012, 48(28), 3442~3444.

14. Guofen Song,Fang Miao, Yuming Sun,Xiaoqiang Yu*,Jing Zhi Sun*, Wai-Yeung Wong*, Fluorescence turn-on probes for intracellularRNA distribution and their imaging in confocal and two-photon fluorescencemicroscopy Sensor. Actuat. B-Chem. 2012, 173, 329~337.

15. Xin Liu,Yuming Sun, Yuanhong Zhang, Fang Miao, Guancong Wang, HongshiZhao,Xiaoqiang Yu*, Hong Liu* and Wai-YeungWong*. A 2,7-carbazole-baseddicationic salt for fluorescence detection ofnucleic acids and two-photon fluorescence imaging of RNA in nucleoli andcytoplasm Org. Biomol. Chem. 2011, 9(10), 3615-3618.

16. Xin Liu,Yuming Sun, Yuanhong Zhang, Ning Zhao, Guancong Wang, HongshiZhao,Xiaoqiang Yu,* Hong Liu* A Series ofCarbazole Cationic Compounds with Large Two-Photon Absorption Cross Sectionsfor Imaging Mitochondria in Living Cells with Two-Photon FluorescenceMicroscopy. J. Fluoresc. 2011, 21(2), 497-506.

17. Zhang Y. H.,Wang J. J., Jia P. F.,Yu X. Q.*,Liu H., Liu X., ZhaoN., Huang B. B. Two-photon fluorescence imaging of DNA inliving plant turbid tissue with carbazole dicationic salt. Org. Biomol. Chem.,2010, 8(20), 4582-4588.

18. ZHAO Ning,ZHANG YuanHong, LIU Xin,YU XiaoQiang*,GE MaoFa. Carbazoletricationic salt: a novel potential two-photon fluorescentDNA probe for nucleic imaging of cells Chin. Sci. Bull. 2010, 55(32),3661-3667.

19. ZHANGYuan-Hong, SUN Yu-Ming, LIU Xin, ZHAO Ning,YU Xiao-Qiang*, HUANG Bai-Biao*. Interaction Between DicationicCarbazole Derivatives and Nucleic Acidby Spectrum. CHEMICALJOURNAL OF CHINESEUNIVERSITIES 2010, 31(9), 1860-1863. ^[1] 

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