劉君

1994.9-1998.6, 南開大學, 微生物學專業, 學士學位

1999.9-2002.6, 南開大學, 生物化學和分子生物學專業，碩士學位

2002.9-2006.1, 中國科學院微生物研究所, 微生物學專業，博士學位

2006.3-2010.6，美國西奈山醫學院，博士後

2010.7-2013.12, 美國西奈山醫學院, 研究助理教授

2014.2-目前，中國科學院天津工業生物技術研究所，研究員 ^[1] 

研究組定位於重要工業微生物的應用基礎研究,圍繞微生物生理和代謝工程開展以下研究:

1、系統解析重要工業微生物對環境脅迫的生理機制，在此基礎上構建具有抗逆性能的工業菌種。

2、闡明重要工業微生物合成特定代謝產物的分子基礎和調控機制，並利用代謝工程和系統生物學等技術實現工業菌種的遺傳改造。 ^[1] 

[1] Wang X, Yang H, Zhou W, Liu J, Xu N.2019. Deletion of cg1360 affects ATP synthase function and enhances the production of L-valine in Corynebacterium glutamicum.J Microbiol Biotechnol. Jul 30. doi: 10.4014/jmb.1904.04019.

[2] Wei L, Wang Q, Xu N, Cheng J, Zhou W, Han GQ, Jiang HF*, Liu J*, Ma YH.2019. High-level o-acetylhomoserine production in Escherichia coli through protein and metabolic engineering. ACS Synthetic Biology, 8(5):1153-1167.

[3] Xu N, Wei L, Liu J*. 2019. Recent advances in the applications of promoter engineering for the optimization of metabolite biosynthesis. World J Microbiol Biotechnol, 35: 33. (Invitedreview)

[4] Wu Z, Wang J, Liu J, Wang Y, Bi C, Zhang X.2019. Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2. Microb Cell Fact. 18(1):15. doi: 10.1186/s12934-019-1067-3.

[5] Xu N, Lv HF, Wei L, Ju JS, Liu J*, Ma YH. 2019. Impaired oxidative stress and sulfur assimilation contribute to acid tolerance of Corynebacterium glutamicum. Appl Microbiol Biotech, doi: 10.1007/s00253-018-09585-y.

[6] Wei L, Wang H, Xu N, Zhou W, Ju JS*, Liu J*, Ma YH. 2019. Metabolic engineering of Corynebacterium glutamicum for L-cysteine production. Appl Microbiol Biotech, doi: 10.1007/s00253-018-9547-7.

[7] Xu N, Zheng YY, Wang XC, Krulwich TA, Ma YH, Liu J*. 2018. The lysine 299 residue endows the multisubunit Mrp1 antiporter with dominant roles in Na-resistance and pH homeostasis in Corynebacterium glutamicum. Appl Environ Microbiol, 84: e00110-18.

[8] Vaish M, Price-Whelan A, Reyes-Robles T, Liu J, Jereen A, Christie S, Alonzo F 3rd, Benson MA, Torres VJ, Krulwich TA.2018. Roles of Staphylococcus aureus Mnh1 and Mnh2 Antiporters in Salt Tolerance, Alkali Tolerance, and Pathogenesis.J Bacteriol.200(5). pii: e00611-17. doi: 10.1128/JB.00611-17.

[9] Wei L, Xu N, Cheng HJ, Wang YR, Han GQ, Ma YH, Liu J*. 2018. Promoter library-based module-combination (PLMC) technology for optimization of threonine biosynthesis in Corynebacterium glutamicum. Appl Microbiol Biotech, 102: 4117-30.

[10] Xu N, Wei L, Liu J*. 2017. Biotechnological advances and perspectives of gamma-aminobutyric acid production. World J Microbiol Biotechnol, 33: 64. (Invitedreview)

[11] Liu QD, Ma XQ, Cheng HJ, Xu N, Liu J*, Ma YH. 2017. Co-expression of L-glutamate oxidase and catalase in Escherichia coli to produce alpha-ketoglutaric acid by whole-cell biocatalyst. Biotechnol Lett, 39 (6):913-9.

[12] Xu N, Wang L, Cheng Hj, Liu Qd, Liu J*, Ma YH. 2016. In vitro functional characterization of the Na/H antiporters in Corynebacterium glutamicum. FEMS Microbiol Lett, 363: fnv237.

[13] Liu QD, Cheng H, Ma X, Xu N，Liu J*, Ma YH 2016. Expression, characterization and mutagenesis of a novel glutamate decarboxylase from Bacillus megaterium. Biotechnol Lett, 38(7): 1107-13.

[14] Preiss L, Langer JD, Hicks DB, Liu J, Yildiz O, Krulwich TA, Meier T. 2014. The c-ring ion binding site of the ATP synthase from Bacillus pseudofirmus？OF4 is adapted to alkaliphilic lifestyle. Mol Microbiol. 92(5):973-84.

[15] Liu J, Ryabichko S, Bogdanov M, Fackelmayer OJ, Dowhan W, Krulwich TA. 2014. Cardiolipin is dispensable for oxidative phosphorylation and non-fermentative growth of alkaliphilic Bacillus pseudofirmusOF4. J Biol Chem. 289(5):2960-71.

[16] Preiss L, Klyszejko AL, Hicks DB,Liu J, Fackelmayer OJ, Yildiz ？, Krulwich TA, Meier T. 2013. The c-ring stoichiometry of ATP synthase is adapted to cell physiological requirements of alkaliphilic Bacillus pseudofirmusOF4. Proc. Natl. Acad. Sci. USA. 110(19):7874-9.

[17] Liu J, Hicks DB, Krulwich TA. 2013. Roles of AtpI and two YidC-type proteins from alkaliphilc Bacillus pseudofirmus OF4 in ATP synthase assembly and non-fermentative growth.J. Bacteriol. 195(2): 220-30.

[18] Janto B, Ahmed A, Ito M, Liu J et al. 2011. The genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4. Environmental Microbiology. 13(12): 3289-3309.

[19] Liu J, Fackelmayer OJ, Hicks DB, Preiss L, Meier T, Sobie EA, Krulwich TA. 2011. Mutations in a helix-1 motif of the ATP synthase c-subunit of Bacillus pseudofirmus OF4 cause functional deficits and changes in the c-ring stability and mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Biochemistry. 50(24):5497-5506.

[20] Fujisawa M, Fackelmayer OJ, Liu J, Krulwich TA, Hicks DB. 2010. The ATP synthase a-subunit of extreme alkaliphiles is a distinct variant: mutations in the critical alkaliphile- specific residue Lys-180 and other residues that support alkaliphile oxidative phosphorylation. J. Biol. Chem. 15; 285(42):32105-15.

[21] Hicks DB, Liu J, Fujisawa M, Krulwich TA. 2010. F1Fo-ATP synthases of alkaliphilic bacteria: lessons from their adaptations. Biochim. Biophys. Acta. 1797(8):1362-1377.

[22] Guo Y, Xue Y,Liu J, Wang Q, Ma Y. 2009. Characterization and function analysis of a Halo-alkaline-adaptable Trk K uptake system in Alkalimonas amylolytica strain N10. Sci China C Life Sci. 52(10):949-57.

[23] Liu J, Fujisawa M, Hicks DB, Krulwich TA. 2009. Characterization of the functionally critical AXAXAXA and PXXEXXP motifs of the ATP synthase c-subunit from an alkaliphilic Bacillus. J. Biol. Chem. 284(13):8714-25.

[24] Liu J, Krulwich TA, Hicks DB. 2008. Purification of two putative type II NADH dehydrogenases with different substrate specificities from alkaliphilic Bacillus pseudofirmus OF4. Biochim. Biophys. Acta. 1777(5):453-61.

[25] Wei Y, Liu J, Ma Y, Krulwich TA. 2007. Three putative cation/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli mutant. Microbiology. 153:2168-2179.

[26] Yuan S, Ren P, Liu J, Xue Y, Ma Y, Zhou P. 2007. Lentibacillus halodurans sp. nov., a moderately halophilic bacterium isolated from a salt lake in Xin-Jiang, China. Int. J. Syst. Evol. Microbiol. 57(3):485-488.

[27] Wang N, Zhang Y, Wang Q, Liu J, Wang H, Xue Y, Ma Y. 2006. Gene cloning and characterization of a novel α-amylase from alkaliphilic Alkalimonas amylolytica. Biotechnol. J. 1(11):1258-65.

[28] Liu J, Xue Y, Wang Q, Wei Y, Swartz TH, Hicks DB, Ito M, Ma Y, Krulwich TA. 2005. The activity profile of the NhaD-type Na(Li)/H antiporter from the soda lake haloalkaliphile Alkalimonas amylolytica is adaptive for the extreme environment. J. Bacteriol. 187(22):7589-95.

[29] Krulwich TA,Liu J, Morino M, Fujisawa M, Ito M, Hicks DB, 2010. Adaptive mechanisms of extreme alkaliphiles. In: Extremophiles Handbook. Horikoshi K, Antranikian G, Bull A, Robb F, Stetter K (eds), Springer, Heidelberg,PP. 120-139. ^[1] 

研究組承擔國家合成生物學重點專項2項,中科院重點部署項目1項, 國家自然科學基金面上項目1項,青年基金3項,天津市自然科學基金2項。 ^[1]